塞来昔布抑制肺癌增殖及血管生成的实验研究  

Experimental study of inhibitive effects of celecoxib on the proliferation of lung cancer cells and angiogenesis

作　　者：刘国华[1] 陈燕明[1] 陈晓军[1] 黄建安[2] 

机构地区：[1]南京医科大学附属苏州市立医院东区呼吸科,江苏苏州215001 [2]苏州大学附属第一医院呼吸科,江苏苏州215006

出　　处：《临床肺科杂志》2012年第11期2042-2043,共2页Journal of Clinical Pulmonary Medicine

基　　金：苏州市科教兴卫青年专项基金(编号:SWQ22)

摘　　要：目的探讨选择性环氧化酶-2(COX-2)抑制剂塞来昔布(西乐葆)对A549肺癌移植瘤的抑制作用及其对肿瘤组织血管生成的影响。方法建立裸鼠肺癌细胞株A549移植瘤模型,药物组给予含1 250 ppm塞来昔布的饮水,连续28 d,计算抑瘤率。采用免疫组化法研究移植瘤血管内皮生长因子(VEGF),微血管密度(MVD)的表达.。结果药物组平均瘤重(0.65±0.20)g,对照组平均瘤重(1.45±0.30)g,两组有显著性差异(P<0.01),塞来昔布抑瘤率为57.23%。药物组与对照组VEGF积分分别为(0.184±0.022)和(0.295±0.032)(P<0.01),MVD平均值分别为(18.80±3.67)和(34.50±5.20)(P<0.01)。结论塞来昔布对A549肺癌移植瘤有明显抑制作用,其作用机制之一与抑制肿瘤血管生成相关。Objective To investigate the effects of eeleeoxib on transplanted A549 lung cancer and angiogenesis. Methods The model of A549 lung adenoeareinoma cell line xenagrafted neoplasia in BALB/C nude mice was made, treatment group began receiving 1250 ppm eeleeoxib in the H20 for 28 days, and all mice were sacrificed on the 28th day after tumor implantation. The expression of VEGF, MVD in xenografted tumor tissue were analyzed with immunohistoehemieal staining. Results The average weight of tumor nodules in treatment group was （0.65 ±0. 15 ）g, while in the control group it was （1.45 ± 0.30）g, and there was statistical difference （P 〈0.01 ）. The inhibitory rate of celecoxib was 57.23 % ; the VEGF staining score of tumor tissue in treatment group and control group were （ 0.184 ± 0. 022） and （0. 295 ± 0. 032 ） （ P 〈 0. 01 ）, respectively, and the average MVD in two groups were （ 18.80 ± 3.67 ） and （ 34.50± 5.20） （ P 〈 0.01 ）, separately. Conclusion Celexocib has inhibitory effects on A549 xenografted neoplasm in nude mice, and inhibition of an- giogenesis might be its important mechanisms.

关 键 词：塞来昔布 肺癌 血管内皮生长因子 微血管密度 

分 类 号：R734.2[医药卫生—肿瘤]