马鹿生长激素(GH)基因生物信息学预测及分析  

Bioinformatics Prediction and Analysis on GH Gene of Cervus elaphus

作　　者：宋兴超[1] 杨福合[1] 刘汇涛[1] 徐超[1] 魏海军[1] 邢秀梅[1] 

机构地区：[1]中国农业科学院特产研究所农业部特种经济动物遗传育种与繁殖重点实验室特种经济动物分子生物学国家重点实验室,长春130112

出　　处：《经济动物学报》2012年第3期133-139,共7页Journal of Economic Animal

基　　金：国家科技支撑计划项目(2011BAI03B02);国家公益性行业(农业)科研专项资助项目(200903014)

摘　　要：为研究马鹿生长激素(GH)基因的结构和功能,从GenBank中下载马鹿、梅花鹿、鼷鹿、牛、山羊、绵羊、猪、人、黑猩猩、挪威大鼠、小家鼠、北极狐、狗、鸡和斑马鱼的GH基因完整编码区(CDS)及氨基酸序列,利用DNAStar 7.0、BioEdit 7.0生物软件与相关在线工具对马鹿GH基因核苷酸序列的基本信息及其编码蛋白的理化特性和结构特征进行了生物信息学预测及分析,对马鹿与其他14个物种GH基因的CDS序列及其编码氨基酸序列进行相似性分析,并基于氨基酸序列构建了15个物种的系统进化树。结果表明:马鹿GH基因DNA序列长度为2 100 bp,包括完整的5个外显子和4个内含子,部分5′UTR和3′UTR,CDS全长654 bp,编码217个氨基酸;其编码的蛋白是一种分子质量为24.588 4 ku,等电点为7.62的疏水性稳定碱性蛋白;存在2个强跨膜区、8个广泛磷酸化位点,二级结构元件以α-螺旋和无规则卷曲为主;该蛋白位于细胞外,含有1个信号肽,属一种分泌型蛋白;马鹿与梅花鹿、牛、绵羊、山羊和鼷鹿等动物的GH基因氨基酸序列相似性较高,亲缘关系最近。该研究结果可为马鹿GH基因的进一步分析提供详细的生物学基础信息。In order to study the structure and function of GH gene of wapiti, coding sequences（CDS） of GH gene in wapiti, sika deer, chevrotain, cattle, goat, sheep, pig, human, chimpanzee, Norway rat, house mouse, arctic fox, dog, chicken and zebrafish were downloaded from GenBank as experimental ma- terials. Bioinformaties analysis was made on basic information and encoding protein structure, physic- chemical property, signal peptide, transmembrane structure, generic phosphorylation sites, secondary structure and subcellular localization were predicted by means of biologic software and online tools. In ad- dition, the similarity of GH gene CDS sequence and amino acid between those of wapiti and other 14 species were also analyzed. Phylogentic tree of the homologous gene based on the amino acid of GH gene was constructed. The results showed that the length of wapiti GH gene was 2 100 bp, which included 5 exons, 4 introns, partial 5＇UTR and 3＇UTR, and it contained an open reading frame of 654 bp, which encoded 217 amino acids. The estimated molecular weight of GH protein was 24.588 4 ku, with a iso- electric point of 7.62 and 31.04 in stability index, belonging to the stable alkalinous protein with hy- drophobicity. The GH protein had two obvious strong transmembrane region, eight phosphorylation sites. The secondary structure of GH protein was mainly α-helix and irregular curly. The extracellular protein contained one signal peptide was probably being secreting type. The similarity comparison and phyloge- netic tree indicated that the evolution distance of wapiti GH gene was the most homogeneous to sika deer, chevrotain, cattle, goat and sheep. The research provided detailed bioinformatics information for further study on GH gene of wapiti.

关 键 词：马鹿 生长激素基因 生物信息学 系统进化 

分 类 号：Q754[生物学—分子生物学] S825[农业科学—畜牧学]