枸杞多酚提取物中总多酚的测定  

Determination of total polyphenols in polyphenol extract from Lycium barbarum

作　　者：黄丽[1] 於洪建[2,3] 吴巍[2] 张颖[2] 

机构地区：[1]天津中医药大学药学院,天津300193 [2]天津尖峰天然产物研究开发有限公司,天津300457 [3]辽宁植物多酚工程技术中心,辽宁沈阳110000

出　　处：《药物评价研究》2012年第4期273-275,共3页Drug Evaluation Research

摘　　要：目的建立紫外分光光度法测定枸杞多酚提取物中的总多酚。方法采用紫外分光光度法测定枸杞多酚提取物中总多酚,用水稀释样品,加入显色剂Filin-Ciocalteu试剂,在760 nm处测定吸光度,用同样方法测定并绘制标准曲线。结果紫外分光光度法测定枸杞多酚提取物中总多酚的量分别为24.03%、26.38%、25.45%、28.27%、29.08%,线性范围1～8μg/mL,R2=0.999 5。平均回收率为103.5%,RSD<5%。结论该方法操作简单,准确可靠,适于枸杞多酚提取物中总多酚的测定。Objective To establish UV-spectrophotometry to determine the content of total polyphenols in polyphenol extract from Lycium barbarum（PELB）.Methods The content of total polyphenols in PELB was detected by using UV-spectrophotometry.The sample was diluted with water,chromogenic agent Filin-Ciocalteu was added,the absorbance was detected at 760 nm by UV-spectrophotometry,and with the same method standard curve was measured and drawn.Results The contents of total polyphenols in different batches of PELB were 24.03%,26.38%,25.45%,28.27%,and 29.08%,respectively,the range of linearity was 1―8 μg/mL,and R2= 0.999 5.The average recovery was 103.5% and the RSD was less than 5%.Conclusion The method is proved to be simple,reliable,accurate,repeatable,and could be used to measure the content of total of polyphenols in PELB.

关 键 词：枸杞多酚提取物 多酚 紫外分光光度计法 

分 类 号：R284.1[医药卫生—中药学]