马铃薯StHb1蛋白的亚细胞定位  

Subcellular localization of StHb1 protein from potato

作　　者：李继刚[1] 郑建坡[1] 曲占良[1] 

机构地区：[1]河北大学生命科学学院,河北省微生物多样性研究与应用重点实验室,河北保定071002

出　　处：《河北大学学报（自然科学版）》2012年第5期523-527,共5页Journal of Hebei University(Natural Science Edition)

基　　金：河北大学博士基金资助项目(2007-097);河北省教育厅资助项目(2007412);河北省科技支撑计划项目(11215529)

摘　　要：为了研究马铃薯StHb1蛋白的亚细胞定位情况,采用RT-PCR技术克隆到马铃薯StHb1基因cDNA序列,并成功构建了StHb1基因与绿色荧光蛋白基因的融合表达载体pBI121-StHb1-GFP.利用农杆菌介导法将重组载体转化洋葱内表皮细胞,通过荧光显微镜观察融合蛋白的瞬时表达以确定StHb1蛋白在细胞内的分布.结果表明StHb1蛋白主要分布于细胞核中.To identify the subcellular location of StHb1 protein,a cDNA encoding nonsymbiotic hemoglobin（StHb1） was cloned from Solanum tuberosum by RT-PCR,and fused to the 5′ end of the green fluorescent protein gene to generate plant binary vector pBI121-StHb1-GFP.It was introduced into onion epidermal cells via Agrobacterium-mediated transformation.Subcellular localization of StHb1 protein was detected by fluorescent microscopy.The results showed that the StHb1-GFP fusion proteins were predominantly present in the nucleus.

关 键 词：非共生血红蛋白 绿色荧光蛋白(GFP) 亚细胞定位 融合表达载体 农杆菌介导转化 

分 类 号：Q789[生物学—分子生物学]