喉癌中PAK1基因mRNA的表达及临床意义  

Expression and its clinical significance of PAK1 mRNA in laryngeal cancer

作　　者：何国庆[1] 管强[1] 张喜军[2] 张杰武[1] 姬宏飞[3] 徐晋[4] 

机构地区：[1]哈尔滨医科大学附属第三医院头颈外科,哈尔滨150081 [2]哈尔滨医科大学附属第四医院耳鼻咽喉科 [3]哈尔滨医科大学肿瘤防治研究所 [4]哈尔滨医科大学基础医学院细胞生物教研室

出　　处：《实用肿瘤学杂志》2012年第5期410-413,共4页Practical Oncology Journal

基　　金：基金项目：黑龙江省教育厅科学技术研究面上项目（11541221）

摘　　要：目的探讨PAK1（p21activated kinase1）基因在喉癌组织中mRNA水平表达情况及临床意义。方法采用实时定量多聚酶链式反应（qRT—PCR）检测喉癌组织和癌旁正常黏膜组织中PAK1基因的mRNA表达情况。结果62例喉癌中PAK1基因mRNA水平的阳性表达率为72．58％（45例），PAK1的表达与肿瘤分级、淋巴结转移、临床分期和不良预后相关（P〈0．05）。结论喉癌中PAK1基因在mR—NA水平表达的上调与喉癌的发生发展密切相关，并且与病理分级、淋巴结转移、临床分期、不良预后有关，表明PAK1可能成为喉癌治疗中的新靶点。Objective To explore the expression level and clinical significance of PAK1 mRNA in laryn- geal squamous cell carcinoma. Methods The mRNA expression level of PAK1 was detected in laryngeal cancer samples and matched para - cancerous normal tissues by real - time quantitative RT - PCR （ qTR - PCR）. Results The positive rate of PAK1 mRNA expression was 72.58% in laryngeal cancer. The high level of PAK1 mRNA was correlated with poor differentiation,lymph node metastasis, advanced clinical stage and poor prognosis （P 〈 0.05）. Conclusion Our study demonstrated that up - regulation of PAK1 mRNA may closely correlated with the carcinogenesis and development of laryngeal cancer. It indicates that PAK1 may serve as a new therapeutic target for laryngeal cancer.

关 键 词：鳞状细胞癌 喉 PAK1 实时定量RT—PCR 

分 类 号：R739.65[医药卫生—肿瘤]