银花泌炎灵片对产ESBLs大肠埃希菌体外抑菌活性研究  

Study on antibacterial activity of Yinhua Miyan Ling Tablet to extended-spectrum β-lactamases-producing E.coli in vitro

作　　者：王槐栋[1] 胡亚[1] 张晓天[1] 王冀邯[1] 黄红兰[1] 王放[1] 倪朝辉[1] 

机构地区：[1]吉林大学白求恩医学院,吉林长春130021

出　　处：《湖南中医药大学学报》2012年第10期3-4,7,共3页Journal of Hunan University of Chinese Medicine

摘　　要：目的研究中药制剂银花泌炎灵片对产ESBLs大肠埃希菌的抑菌活性。方法对40株大肠埃希菌临床株进行产ESBLs表型筛选和确认,采用K-B(Kirby-Bauer)纸片扩散法进行药敏实验,采用2倍平板稀释法测定银花泌炎灵片对产ESBLs大肠埃希菌的体外最低抑菌浓度(MIC)。结果在40株大肠埃希菌临床分离株中检出产ESBLs菌株18株(检出率45.0%),所有产ESBLs株均为多重耐药菌株,对氨苄西林、头孢唑啉、哌拉西林、头孢呋辛酯、复方新诺明、头孢克洛的耐药率均超过90%,对亚胺培南、哌拉西林/他唑巴坦,阿米卡星、阿莫西林/克拉维酸、头孢吡肟及头孢西丁的耐药率较低,均在30%以下。银花泌炎灵片对产ESBLs大肠埃希菌临床株及质控对照株ATCC25922和ATCC35218的MIC均为200mg/mL～100 mg/mL,抑菌活性差异无统计学意义(P>0.05)。结论银花泌炎灵片对产ESBLs大肠埃希菌具有较强的体外抑菌作用。Objective To study the antibacterial effect of Yinhua Miyan Ling Tablet to ESBLs-producing E.coli in vitro.Methods ESBLs-producing strains were screened and confirmed from 40 E.coli clinical strains.K-B（Kirby-Bauer） diffusion method was used to detect the antibiotic resistance.The minimum inhibitory concentrations（MICs） of Yinhua Miyan Ling Tablet to ESBLs-producing Escherichia coli and Escherichia coli ATCC25922、ATCC35218 were determined by standard plate dilution method in vitro.Results 18 ESBLs-producing strains were confirmed among 40 clinical isolates（detection rate was 45.0%）.All ESBLs-producing strains were multiple resistant strains.The resistance rates to ampicillin,cefazolin,piperacillin,cefuroxime axetil and cefaclor were more than 90%.The resistance rates to imipenem,piperacillin/tazobactam,amikacin,amoxicillin/clavulanic acid,cefepime,and cefoxitin were lower,in less than 30%.The MIC of Yinhua Miyan Ling Tablet to ESBLs-producing Escherichia coli clinical stains and standard control strains ATCC25922 and ATCC35218 were all between 200mg/mL-100mg/mL.And there was no significant difference（P0.05）.Conclusion Yinhua Miyan Ling Tablet has a strong antibacterial activity to extended-spectrum β-lactamases-producing E-coli in vitro.

关 键 词：银花泌炎灵片 Β-内酰胺酶 大肠杆菌 抑菌活性 最低抑菌浓度 

分 类 号：R285[医药卫生—中药学]