Exploring Quaternized Hydroxyethylcellulose as Potential Gene Carriers  

作　　者：丁菲 尤俊 翁小成 周金平 章晓联 周翔 张俐娜 

机构地区：[1]College of Chemistry and Molecular Sciences,Key Laboratory of Biomedical Polymers of Ministry of Education,State Key Laboratory of Virology,Wuhan University,Wuhan,Hubei 430072,China [2]School of Medicine,Wuhan University,Hubei,Wuhani 430071,China [3]State Key Lab ofBioorganic&Natural Product Chemistry,Shanghai Institute of Organic Chememistry,Shanghai 200032,China

出　　处：《Chinese Journal of Chemistry》2012年第9期2212-2218,共7页中国化学（英文版）

摘　　要：Cationic polysaccharides have been receiving more attentions and used as nonviral gene delivery vectors. In this paper, quaternized hydroxyethylcellulose （QHEC） derivatives were studied as gene carriers for their efficient DNA binding abilities. All QHECs could form stable QHEC/DNA complexes and resist the degradation of DNase I. And the dynamic light scatter （DLS） results showed that all QHEC/DNA complexes could form compact particles. These QHEC/DNA complexes exhibited effective transfeetion abilities in comparison to the naked DNA. The cytotox- icities of QHEC and QHEC/DNA complexes were also evaluated in four cell lines which were relatively low com- pared with 25 kDa bPEI. All results indicated that these quaternized hydroxyethylcelluloses could be used as poten- tial gene delivery vectors.Cationic polysaccharides have been receiving more attentions and used as nonviral gene delivery vectors. In this paper, quaternized hydroxyethylcellulose （QHEC） derivatives were studied as gene carriers for their efficient DNA binding abilities. All QHECs could form stable QHEC/DNA complexes and resist the degradation of DNase I. And the dynamic light scatter （DLS） results showed that all QHEC/DNA complexes could form compact particles. These QHEC/DNA complexes exhibited effective transfeetion abilities in comparison to the naked DNA. The cytotox- icities of QHEC and QHEC/DNA complexes were also evaluated in four cell lines which were relatively low com- pared with 25 kDa bPEI. All results indicated that these quaternized hydroxyethylcelluloses could be used as poten- tial gene delivery vectors.

关 键 词：cationic cellulose DNA gene carrier TRANSFECTION 

分 类 号：Q782[生物学—分子生物学] TQ352.72[化学工程]