柔红霉素对K562细胞凋亡及FAKmRNA影响的研究  

Effects of DNR on cell apoptosis and expression of fakmRNA in K562

作　　者：王英[1] 严海燕[1] 林向华[1] 罗金刚[1] 许英[1] 史沛杰[1] 

机构地区：[1]中山大学孙逸仙纪念医院检验科,510120

出　　处：《中国实用医药》2012年第27期1-3,共3页China Practical Medicine

摘　　要：目的研究柔红霉素诱导K562细胞增殖和凋亡情况,以及调节细胞周期和FAKmRNA基因,进一步探讨通过调控FAK表达,研究抗白血病的作用机制。方法应用CCK8细胞增殖法,结合流式细胞仪检测法检测不同浓度柔红霉素在不同时间对K562细胞增殖和凋亡的影响,应用RT-PCR和Westernblot技术检测不同浓度柔红霉素作用对K562细胞FAKmRNA以及蛋白表达水平的变化。结果 K562细胞的增殖抑制率随着柔红霉素浓度增加及作用时间延长逐渐升高,同一浓度不同时间组之间,或者同一时间不同浓度组之间比较,差异均有统计学意义(P<0.05);柔红霉素能引起K562细胞凋亡,且随着药物浓度增加,凋亡率也逐渐增加,差异均有统计学意义(P<0.05);柔红霉素能引起K562细胞周期阻滞,多停留在S期;柔红霉素能引起K562细胞FAKmRNA表达和FAK蛋白表达水平的降低。结论一定浓度的柔红霉素在体外可诱导细胞凋亡增加并抑制分裂期细胞的增殖,对细胞FAK基因和蛋白水平都有显著下调,发生凋亡的机制可能是通过抑制FAK基因表达。Objective To investigate the effects of DNR on proliferation,cell cycle,apoptosis and the expression of FAKmRNA of human chronic mye loidleukemia （CML） cell line K562,and futher to explore the mechanism of regulating FAK to against leukemia.Methods The CCK8 method and the flowcytometry was used to detect the effects of DNR in various concentrations and at different time points on proliferation and apoptosis of K562 cells,the RT-PCR and Western blot was used to assay the expression of FAKmRNA and FAK protein in K562 cells treated with DNR in various concentrations.Results Along with increasing of the concentration and prolonging of time,the inhibitory rate of DNR on K562 proliferation was gradually enhanced.The comparison between various time groups in the same concentration or various concentration groups at same time showed significant differences （P〈 0.05）.DNR can induce the apoptosis of K562 cells and the comparison between various concentration groups showed significant differences （P〈 0.05）.DNR made K562 cell cycle blocked and most cells blocked in S phase.DNR can decrease the expression levels of fakmRNA and FAK proteins in K562 cells.Conclusion It suggested from the rsesults that at certain concentrations,DNR can induce the apoptosis of K562 cells in vitro.And K562 cells can be efficiently retardanted in division phase.FakmRNA and FAK protein levels can be significantly regulated by DNR.The mechanism was supposed by suppressing the expression of FAK gene.

关 键 词：白血病 K562细胞 柔红霉素 细胞增殖 凋亡 FAKmRNA 

分 类 号：R733.7[医药卫生—肿瘤]