Zinc Finger Protein-activating Transcription Factor Up-regulates Vascular Endothelial Growth Factor-A Expression in Vitro  

作　　者：Li-shan Lian Yao-guo Yang Wei Liu Li-long Guo Heng Guan Chang-wei Liu Yong-jun Li 

机构地区：[1]Department of Vascular Surgery,Peking Union Medical College Hospita Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100730,China

出　　处：《Chinese Medical Sciences Journal》2012年第3期171-175,共5页中国医学科学杂志（英文版）

基　　金：Supported by the National Natural Science Foundation of China(81270399and81100226)

摘　　要：Objective To construct the zinc finger protein-activating transcription factor (ZFP-ATF) plasmid and evaluate its efficacy in inducing vascular endothelial growth factor (VEGF) expression in EY.HY926 endothelial cells. Methods Firstly, we constructed the ZFP-ATF plasmid, then testified the quantity of VEGF protein in EY.HY926 endothelial cells after transfected with ZFP-ATP plasmid by Western blot, finally, we used the RT-PCR to testify whether the ZFP-ATF can stimulate expression of VEGF splice variants. Results The ZFP-ATF DNA sequences were located the multiclone sites of PVAX1 vector between the site of BamH Ⅰ and Xhol. Western blot result showed VEGF expression in EY.HY926 endothelial cells transfected with ZFP-ATF plasmid was significantly higher than that in cells transfected with VEGF165 (19.95±3.95 vs. 12.15±1.55 μg/μL, P<0.01). RT-PCR result showed VEGF-A mRNA expression level induced by ZFP-ATF was high than that induced by VEGF165. Conclusion ZFP-ATF can up-regulate the VEGF-A expression in comparison with VEGF165, which might have beneficial effects in angiogenesis process.

关 键 词：zinc finger protein-activating transcription factor vascular endothelial growth factor ANGIOGENESIS 

分 类 号：Q78[生物学—分子生物学] Q513.1