Effects of Zhichan powder on signal transduction and apoptosis-associated gene expression in the substantia nigra of Parkinson's disease rats  

作　　者：Jiajun Chen Jinshu Ma Yafei Qiu Shihong Yi Yongmao Liu Qingwei Zhou PengguoZhang Quan Wan Ye Kuang 

机构地区：[1]Department of Neurology, China-Japan Union Hospital, Jilin University, Changchun 130031, Jilin Province, China [2]Department of Immunology, Norman Bethune College of Medicine, Jilin University, Changchun 130021, Jilin Province, China [3]Department of Biochemistry and Molecular Biology, Institute for Regenerative Medicine, Jilin University, Changchun 130021, Jilin Province,China [4]Department of Image, Second Clinical Hospital, Jilin University, Changchun 130021, Jilin Province, China [5]Department of Biotechnology, Life Science College, Jilin University, Changchun 130012, Jilin Province, China

出　　处：《Neural Regeneration Research》2012年第27期2115-2122,共8页中国神经再生研究（英文版）

摘　　要：Previous studies have shown that Zhichan powder elevated immunity and suppressed oxidation in mice. Rat models of Parkinson＇s disease were induced by stereotaxically injecting 6-hydroxydopamine into the substantia nigra. The rat models were intragastrically treated with Zhichan powder, which is composed of milkvetch root, ginseng, bunge swallowwort root, himalayan teasel root. Magnolia officinalis, Ligustrum lucidum Ait. and szechwan Iovage rhizome. Immunohistochemistry and reverse transcription-PCR results demonstrated that mRNA and protein expression of tumor necrosis factor receptor 1, Fas, caspase-8, cvtochrome C, Bax, casDase-3, and p53 significantly increased, but Bcl-2 expression significantly decreased in the substantia nigra of rats with Parkinson＇s disease. Following Zhichan powder administration, mRNA and protein expression of tumor necrosis factor receptor 1, Fas, caspase-8, cytochrome C, Bax, caspase-3, and p53 diminished, but Bcl-2 expression increased in the rat substantia nigra. These results indicate that Zhichan powder regulates signal transduction protein expression, inhibits apoptosis, and exerts therapeutic effects on Parkinson＇s disease.Previous studies have shown that Zhichan powder elevated immunity and suppressed oxidation in mice. Rat models of Parkinson＇s disease were induced by stereotaxically injecting 6-hydroxydopamine into the substantia nigra. The rat models were intragastrically treated with Zhichan powder, which is composed of milkvetch root, ginseng, bunge swallowwort root, himalayan teasel root. Magnolia officinalis, Ligustrum lucidum Ait. and szechwan Iovage rhizome. Immunohistochemistry and reverse transcription-PCR results demonstrated that mRNA and protein expression of tumor necrosis factor receptor 1, Fas, caspase-8, cvtochrome C, Bax, casDase-3, and p53 significantly increased, but Bcl-2 expression significantly decreased in the substantia nigra of rats with Parkinson＇s disease. Following Zhichan powder administration, mRNA and protein expression of tumor necrosis factor receptor 1, Fas, caspase-8, cytochrome C, Bax, caspase-3, and p53 diminished, but Bcl-2 expression increased in the rat substantia nigra. These results indicate that Zhichan powder regulates signal transduction protein expression, inhibits apoptosis, and exerts therapeutic effects on Parkinson＇s disease.

关 键 词：Zhichan powder Parkinson＇s disease 6-HYDROXYDOPAMINE signal transduction APOPTOSIS substantia nigra traditional Chinese medicine degenerative disease neural regeneration 

分 类 号：Q26[生物学—细胞生物学] Q593.2