白介素-8对体外培养黑素细胞的影响  

Effects of Interleukin-8 on Human Melanocytes in Vitro

作　　者：陈静宇[1] 林新瑜[2] 王芳[2] 林鸿刚[3] 

机构地区：[1]成都市第六人民医院皮肤科,四川成都610051 [2]四川省医学科学院四川省人民医院皮肤病性病研究所,四川成都610072 [3]四川大学国家生物治疗重点实验室,四川成都610041

出　　处：《贵阳医学院学报》2012年第5期500-503,506,共5页Journal of Guiyang Medical College

基　　金：四川省卫生厅资助课题[110219]

摘　　要：目的:建立正常人表皮黑素细胞体外培养体系,观察不同浓度白介素-8对体外培养正常人黑素细胞的细胞增殖及黑素合成的影响。方法:采用四甲基偶氮唑盐(MTT)法测定不同浓度白介素-8对黑素细胞增值的影响,NaOH裂解法测定黑素生成量,流式细胞仪检测黑素细胞的凋亡率。结果:白介素-8对黑素细胞活力有增强作用,能使细胞增殖能力增加,黑素合成增加,但对黑素细胞的凋亡率无显著性影响。结论:白介素-8对体外培养的黑素细胞增殖及黑素生成都有增强作用。Objective： To establish the culture system of human normal melanocytes in vitro, and to study the effects of interleukin-8 at different concentrations on the proliferation of human melanocytes and melanin synthesis. Methods： MTT method and NaOH lysis method were employed to measure the effects of interleukin-8 at different concentrations on the proliferation of human melanocytes and mela- nin respectively. Apoptosis rates of melanocytes were detected with flow cytometer. Results： Interleu- kin-8 promotes melanocyte proliferation and melanin synthesis. Conclusions： Interleukin-8 enhances melanocyte proliferation and melanin synthesis.

关 键 词：白细胞介素-8 黑素细胞 细胞培养 

分 类 号：R36[医药卫生—病理学] R751[医药卫生—基础医学]