核酸内切酶在肝门部胆管癌组织和细胞株中的表达和临床意义  

Expression and clinical significance of dicer in hilar cholangiocarcinoma tissues and cells

作　　者：杨光耀[1] 杨康[1] 温松奇[1] 俞亚红[1] 丁志强[1] 陈勇军[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院胆胰外科,武汉430030

出　　处：《中华肝胆外科杂志》2012年第10期753-757,共5页Chinese Journal of Hepatobiliary Surgery

基　　金：国家自然科学基金面上项目（No.81071998）;湖北省自然科学基金项目（No.2008CDB]59）;教育部高等学校博士学科点专项科研基金（No.20070487114）;华中科技大学自主创新基金（2011JC035）

摘　　要：目的研究核酸内切酶（Dicer）在肝门部胆管癌组织和胆管癌细胞株（QBC939）中的表达及其与临床病理因素和预后的相关性。方法采用免疫组化染色检测40例肝门部胆管癌和10例正常胆管组织中Dicer的表达；Western—blot和RT—PCR检测人胆管癌细胞株QBC939与人胆管上皮细胞株HIBEpic中Dicer的表达量；分析Dicer表达与临床病理因素的相关性。对根治性切除的肝门部胆管癌患者术后总生存期和无病生存期进行单因素和多因素分析。结果Dicer在肝门部胆管癌组织中的表达明显低于正常胆管组织（P〈0．05），在QBC939中的表达明显低于HIBEpic（P〈O．05），高分化胆管腺癌中Dicer表达明显高于中、低分化腺癌。单因素生存分析结果显示Dicer低表达组的根治性切除术后总生存期和无病生存期均短于高表达组（P〈0．01）。多因素分析显示Dicer表达水平为与预后相关的独立危险因素（P〈0．05）。结论在肝门部胆管癌和胆管癌细胞株Dicer表达下调；低表达Dicer的胆管癌患者预后更差。Dicer可能为肝门部胆管癌的诊断和判断预后的分子标志物。Objective To investigate the relationship between Dicer expression and clinicopatho- logical characteristics and prognosis by detecting the expression of Dicer in hilar cholangiocarcinoma tissues and cells. Methods The expression of Dicer in tissues was detected using immunohistochemis- try. Western blotting and RT-PCR were used to investigate Dicer expression in QBC939 and HIBEpic cells. The relationship between Dicer expression and clinicopathological characteristics was analyzed. A Kaplan-Maier analysis was performed to analyze the disease-free survival （DFS） and overall survival （OS） after radical surgical resection of hilar cholangiocarcinoma. Results When compared to control, Dicer was significantly down-regulated in hilar cholangiocarcinoma tissues （P〈O. 05） and in QBC939 （P〈0.05）. The expression of Dicer was higher in well differentiated adenocarcinoma than poorly and moderately differentiated tumours. Univariate analysis showed low expression of Dicer protein was significantly correlated with short disease-free survival and overall survival of patients with hilar eholangiocarcinoma after radical surgical resection （P〈0.01）. Multivariate analysis revealed that the expression of Dicer was the most important factor for predicting prognosis after radical surgical resec- tion of hilar eholangiocarcinoma （P〈0.05）. Conclusions Dicer could be used as a prognostic marker for hilar cholangiocarcinoma.

关 键 词：DICER 肝门部胆管癌 临床病理因子 预后 

分 类 号：R735.8[医药卫生—肿瘤]