IRX1基因在肝癌中的表达及其临床意义  

Expression and clinical significance of Iroquois homebox gene IRX1 in human hepatocellular carcinoma cells

作　　者：杨惠洁[1] 余爽[1] 王静[1] 陈楚[1] 顾颖[1] 鲍依稀[1] 

机构地区：[1]重庆医科大学附属第二医院检验科,重庆400010

出　　处：《中国生物制品学杂志》2012年第10期1354-1357,共4页Chinese Journal of Biologicals

基　　金：重庆市科委课题项目(CSCT 2009BB5399);重庆市教委课题项目(KJ110303)

摘　　要：目的探讨易洛魁家族同源盒基因IRX1(Iroquois homebox gene)在肝癌中的表达及其临床意义。方法收集82份肝癌组织(高分化26份,Edmondson分级Ⅰ级;中分化25份,Edmondson分级Ⅰ-Ⅱ和Ⅱ级;低分化31份,Edmondson分级Ⅱ-Ⅲ和Ⅲ级)和11份正常或良性疾病非癌组织标本,采用实时荧光定量PCR(Q-PCR)和Western blot检测各组肝脏组织中IRX1的表达,免疫组织化学法检测IRX1在肝脏组织中的定位及临床特征。结果肝癌组织中IRX1基因和蛋白的表达均明显高于非癌对照组(P<0.05),且其表达量与肿瘤的分化程度相关,肿瘤分化程度越低,其表达量越高(P<0.05)。IRX1在肝癌组织中的阳性表达率明显高于非癌对照组(P<0.05),且伴随着肿瘤分期增加和分化程度的降低,IRX1阳性率越高,但IRX1阳性率与HBV感染以及伴随肝硬化情况无明显相关性(P>0.05)。IRX1染色阳性的高分化肝癌组织中,68.2%定位于细胞质,18.2%定位于细胞核,13.6%胞质和胞核均阳性;IRX1染色阳性的低分化肝癌组织中,胞质胞核均阳性的增加至65.5%。结论IRX1可能参与调控肝癌的发生发展过程,IRX1的表达与肝癌的分化程度有关,提示IRX1可作为判断肝癌预后、分化程度以及肿瘤靶向分子治疗的指标。Objective To investigate the expression and clinical significance of Iroquois homebox gene IRX1 in human hepatoeellular carcinoma （HCC） cells. Methods A total of 82 hepatocellular carcinoma tissue samples were collected, of which 26 were highly differentiated tissues at Edmondson stage I , 25 were moderately differentiated tissues at Edmondson stages Ⅰ-Ⅱ and Ⅱ, 31 were lowly differentiated tissues at Edmondson stage Ⅲ , and determined for expression of IRX1 by real-time fluorescent quantitative PCR （Q-PCR） and Western blot, and for location of IRX1 and clinical character by immunohistochemistry, using 11 normal or non-tumor tissue samples as control. Results Both the expression levels of IRX1 gene and protein were higher in hepatocellular carcinoma tissue than in control tissue （P 〈 0. 05）, which were related to the differentiation level of carcinoma. The lower the differentiation level of hepatocellular carcinoma tissue was, the higher the expression level of IRX1 was （P 〈 0. 05）. The positive rate of IRX1 in hepatocellular carcinoma tissue was significantly higher than that in non-tumor control tissues, which increased with the increased stage and decreased differentiation level of carcinoma, while showed no significant relationship to HBV infection or accompanied liver cirrhosis （P 〉 0. 05）. Of the highly differentiated carcinoma tissues positive for IRX1, 68. 2% were positive in nuclei, 18. 2% in cytoplasm, and 13. 6% in both nuclei and cytoplasm. However, of the lowly differentiated carcinoma tissues positive for IRX1, 65. 5% were positive in both nuclei and cytoplasm. Conclusion IRX1 might involve in the regulation of onset and progress of HCC, of which the expression was related to the differentiation of carcinoma, indicating that IRX1 might be used as an indicator for evaluation of prognosis and differentiation level as well as targeted molecular therapy of HCC.

关 键 词：IRX1基因 肝癌 分化 

分 类 号：R735.7[医药卫生—肿瘤]