疏肝健脾方对腹泻型肠易激综合征大鼠血浆及结肠组织5-HT、SP、VIP的影响  

Effects of Shugan Jianpi prescription on 5-HT,SP and VIP content in the plasma and colon of D-IBS rat

作　　者：汪正芳[1] 郭前坤[1] 张声生[1] 

机构地区：[1]首都医科大学附属北京中医医院消化中心,北京100010

出　　处：《天津中医药》2012年第5期459-462,共4页Tianjin Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(30973746);北京市自然科学基金资助项目(7102084);北京市教委科技发展计划资助项目(KM201010025027)

摘　　要：[目的]观察疏肝健脾方对腹泻型肠易激综合征大鼠血浆及结肠组织5-羟色胺(5-HT)、P物质(SP)和血管活性肠肽(VIP)的影响。[方法]将60只SD大鼠按随机数字表法随机分为正常组、模型组、得舒特组、疏肝健脾方低、中、高剂量组,使用乙酸灌肠加束缚的方法建立腹泻型肠易激综合征(D-IBS)大鼠模型,应用免疫组化法检测5-HT、SP及VIP。[结果]疏肝健脾方可以明显降低大鼠的粪便含水量(P<0.05);疏肝健脾方中、高剂量组腹部撤退反射(AWR)评分在不同扩张容量下均较模型组明显降低(P<0.05);疏肝健脾方可以降低大鼠血浆中5-HT和结肠组织中SP的含量,而血浆中SP、VIP及结肠组织中VIP含量无显著变化。[结论]疏肝健脾方可以改善大鼠的腹泻及内脏的高敏感性,并对5-HT及SP有调节作用,提示疏肝健脾方可以有效治疗D-IBS,并与调节胃肠激素有关。[Objective] To observe the effect of Shugan Jianpi prescription （SGJP） on the concentration of 5-HT, SP and VIP content in the plasma and colon of D-IBS rat. [Methods] Sixty SD male rats were randomly divided into six groups with ten rats each： normal group, D-IBS model group, pinaverium bromide group and SGJP low, middle, high dosage group. D-IBS rat model was induced by intracolonic instillation of acetic acid and restraint stress and immunohistochemistry technology was employed to test the 5-HT, SP and VIP content in the plasma and colon of rat. [Results] The defecation moisture was significantly reduced by the SGJP prescription （P〈0.05）; the AWR scores of the D-IBS rats under different expansion volume were reduced after treated with the middle and high dosage of SGJP prescription （P〈0.05）; the content of plasma 5-HT and colon SP of D-IBS rats was reduced after administration with SGJP prescription, but plasma SP, VIP and colon VIP were not influenced. [Conclusion] The SGJP prescription could improve the diarrhea and visceral hypersensitivity and regulate the content of 5-HT and SP, which shows that the SGJP prescription could efficiently treat D-IBS by regulating gut hormodes.

关 键 词：疏肝健脾方 腹泻型肠易激综合征 胃肠激素 5-羟色胺 P物质 血管活性肠肽 

分 类 号：R57[医药卫生—消化系统]