代谢综合征模型的建立及其靶器官改变  

ESTABLISHMENT OF ANIMAL MODEL OF METABOLIC SYNDROME AND CHANGES OF CRITICAL ORGANS

作　　者：蓝忠[1] 龙惠东[1] 曾昭华[1] 罗碧辉[1] 张海军[1] 苏子焯[1] 刘宝骅[1] 杜一鹏[1] 

机构地区：[1]广州医学院第一附属医院,广州510120

出　　处：《现代预防医学》2012年第3期725-727,732,共4页Modern Preventive Medicine

基　　金：广东省科技计划项目(2009B030801359);广东省自然基金(9151018201000020)

摘　　要：[目的]建立代谢综合征动物模型并观察其靶器官相关改变。[方法]36只雄性SPF级3周龄雄性Wis-tar大鼠,随机分成3组:正常盐组(NS,n=12)、高盐组(HS,n=12)及高盐高脂组(HSF,n=12)。实验结束,测量体重、颈总动脉有创血压、血钾、血钠、血糖(FBG)、血脂、胰岛素(INS)浓度及胰岛素抵抗指数(HOMA-IR)、病理学检查胸主动脉内膜增厚指标、胰岛素表达以及胰岛β细胞凋亡检测。[结果]实验结束时,高盐高脂组大鼠表现出典型MS特征并观察到胸主动脉内膜增厚、胰腺组织的β细胞表达下降以及胰腺β细胞凋亡等改变。(1)体重增加:HSF组体重明显增加,与NS及HS组比较,P﹤0.05。(2)血压升高:HSF组收缩压均明显升高,与NS组比较,P﹤0.01;HSF组与HS组之间无明显差异。(3)血脂异常:HSF组较NS组大鼠血脂明显增高,以TC、TG、LDL-C为主,三者均P﹤0.05,HDL-C无明显改变(P﹥0.05),HS组与NS组之间无明显差异。(4)胰岛素抵抗:HFS组大鼠胰岛素抵抗指数较NS组明显升高(P﹤0.01),HS组与NS组之间无明显差异;(5)胸主动脉内膜增厚指数(I/M):HSF组及HS组I/M比值高于NS组(0.219±0.050),P﹤0.05;HSF组I/M比值高于HS组,P﹤0.05。(6)免疫组化检测提示与NS组比较,HFS组大鼠胰腺组织的β细胞表达下降。(7)TUNEL胰岛β细胞凋亡检测提示与NS组比较,HFS组细胞凋亡明显增加。[结论]实验结果提示,通过高盐高脂饲料喂养3周龄雄性Wistar大鼠能够诱导形成MS模型,并且可以观察到HFS组大鼠胸主动脉内膜增厚及其胰腺组织β细胞表达下降、胰岛β细胞凋亡明显增加等改变。[Objective]To establish an animal model of metabolic syndrome and observe the changes of critical organs.[Methods]36 male specified-pathogens free（SPF）Wistar rats,aged 3 weeks,were divided into three groups in randomized manner：（1）control with normal salt diet（0.5% NaCl,NS group）;（2）high salt diet（4% NaCl,HS group）;（3）fat and salt-enriched diet（49%fat＋4%NaCl,HSF group）,12 animals in each group.Then the bodyweights were measured,the right carotid artery was calculated for direct blood pressure measurement;detected blood glucose,blood-fat,concentration of Insulin（INS）,insulin resistance index（HOMA-IR）,thickening of tunica intima of aorta pectoralis by pathology,islet insulin expression and apoptosis of beta cell by enzyme linked immunosorbent sorbent assay（ELISA）.[Results]（1）Body weight significantly increased in HSF group,compared with NS group and HS group（P﹤0.050）;（2）The systolic pressure in HSF group significantly improved compared with NS group（P﹤0.01）,was not different with HS group;（3）Comparing with NS group,TC,TG,LDL-C in HSF group significantly increased（P﹤0.05）,but HDL-C was not significantly different（P﹥0.05）;and TC,TG,LDL-C and HDL-C in HSF group were not significantly different with HS group;（4）Insulin resistance index was higher in HSF group than in NS group（P﹤0.01）,but there was no significant difference in HS group and NS group;（5）Thickening index（I/M）of tunica intima of aorta pectoralis was higher in and HSF and HS groups than NS group,I/M in HSF group was higher than in HS group（P﹤0.05）;（6）beta cell of islet insulin expression in HSF group decreased more than NS group;（7）Cell apoptosis of beta cell in HSF group obviously increased compared with NS group.[Conclusion]The fat and salt-enriched diet given to rats could induce metabolic syndrome,which corresponds or is analogous to the clinical metabolic syndrome;and it also leads to thickening of tunica intima of aorta pectoralis,decrease of

关 键 词：代谢综合征 高盐 高脂 血管内膜 胰岛素抵抗 

分 类 号：R-332[医药卫生]