环氧化酶-2抑制剂塞莱昔布对急性白血病细胞VEGF、b-FGF和TGF-β mRNA表达的影响  

Effects of COX-2 Inhibitor Celecoxib on Expressions of VEGF,b-FGF and TGF-β mRNA in Acute Leukemia Cells

作　　者：张彦芳[1] 阮林海[1] 赵小强[1] 

机构地区：[1]河南科技大学第一附属医院血液内科,河南洛阳471003

出　　处：《中国实验血液学杂志》2012年第5期1086-1089,共4页Journal of Experimental Hematology

基　　金：河南省卫生厅科技攻关项目(编号201003081)

摘　　要：本研究旨在探讨环氧化酶-2(COX-2)特异性抑制剂塞莱昔布(celecoxib)对急性白血病细胞血管新生因子血管内皮生长因子(VEGF)、碱性成纤维细胞生长因子(b-FGF)和转化生长因子-β(TGF-β)mRNA表达的影响及其意义。采用半定量逆转录多聚酶链反应(RT-PCR)法检测30例急性白血病细胞经塞莱昔布干预前、后VEGF、b-FGF和TGF-βmRNA表达及变化。结果表明,急性白血病细胞存在VEGF、b-FGF和TGF-βmRNA的表达。VEGFmRNA与b-FGF mRNA表达存在正相关(r=0.559,P=0.001),VEGF mRNA与TGF-βmRNA表达存在负相关(r=-0.4,P=0.029)。经塞莱昔布(80μmol/L,48 h)处理后急性白血病细胞的VEGF、b-FGF和TGF-βmRNA表达较对照组明显下降,差异有统计学意义(P<0.01)。结论:塞莱昔布通过下调血管新生因子的表达而抑制血管增生,对急性白血病治疗具有辅助作用。This study was aimed to investigate the influence and significance of celecoxib（specific inhibitor of cycloxygenase-2） on mRNA expressions of vascular endothelial growth factor（VEGF）,basic fibroblast growth factor（b-FGF）,transforming growth factor β（TGF-β） in acute leukemia cells.The expressions of VEGF,b-FGF,TGF-β mRNA were measured by RT-PCR in acute leukemia cells treated with celecoxib（80 μmol/L,for 48 h） or with PBS.The results showed that the obvious expressions of VEGF,b-FGF,TGF-β mRNA were observed in acute leukemia cells.By using Pearson correlation analysis,there was positive correlation between VEGF mRNA and b-FGF mRNA expressions（r=0.559,P=0.001）,and negative correlation between VEGF and TGF-β mRNA expressions（r=-0.4,P= 0.029）.Expression levels of VEGF,b-FGF,TGF-β mRNA in experimental group were lower than that in control group（P0.01）.It is concluded that COX-2 inhibitor celecoxib can inhabit vascular endothelial growth through down-regulating the mRNA expression of VEGF,b-FGF and TGF-β in acute leukemia cells.COX-2 inhibitor may offer supplemental effect for treating acute leukemia.

关 键 词：急性白血病细胞 塞莱昔布 VEGF B-FGF TGF-Β 

分 类 号：R733.71[医药卫生—肿瘤] R949.1[医药卫生—临床医学]