不同器官石蜡包埋切片的切片压缩对比研究  

Comparison of the compressions of paraffin-embedded sections from different organs

作　　者：彭彬[1] 陈茂山[2] 李惠[2] 杨正伟[3] 

机构地区：[1]川北医学院细胞化学研究室,南充637007 [2]川北医学院临床医学系,南充637007 [3]川北医学院形态定量研究室,南充637007

出　　处：《中国体视学与图像分析》2012年第3期219-225,共7页Chinese Journal of Stereology and Image Analysis

基　　金：四川省教育厅项目(10ZA176)

摘　　要：目的比较研究不同器官石蜡包埋切片的切片压缩。方法从一成年雄性SD大鼠的肺、心、肝、睾丸和脊髓腰膨大各切取3个长、宽、高均约为2 mm的组织块,石蜡包埋后从每个组织块切取5～40μm厚(切片机设定厚度)的切片各3张进行苏木精染色,然后利用体视学图像系统的指针测微计测量每张切片染色后的实际厚度,以后一厚度(实际厚度)与前一厚度(切片设定厚度)之比作为切片压缩系数。结果与结论不同器官的平均压缩系数分别为84.4%(肺)、81.4%(心脏)、80.6%(肝脏)、77.9%(睾丸)和72.2%(脊髓),不同器官间有显著性差异。Objective To compare the compressions of paraffin-embedded sections from various organs. Methods Three tissue blocks, of length, width and height of approximately 2 ram, were obtained from the lung, heart, liver, testis and spinal cord lumbar enlargement in an adult male SD rat, respectively, and then processed by paraffin embedding. Three sections of thickness （ set by microtome） of 5 - 40 μm were cut from each tissue block and stained with hematoxylin. The actual thickness of each section after staining was measured using a microcator equipped in a stereology image analysis system. The coefficient of section compression was estimated by dividing the latter thickness （ actual thickness） by the former one （initial thickness at sectioning）. Results and conclusions The compression coefficients of sections from different organs were 84. 4% （lung）, 81.4% （heart）, 80. 6% （liver）, 77.9% （testis） and 72.2% （spinal cord） , with significant differences between organs.

关 键 词：石蜡切片 切片厚度 切片压缩 体视学 大鼠 

分 类 号：R361[医药卫生—病理学]