高效降酚菌株Ochrobactrum sp. CH10生长动力学和苯酚降解特性的研究  

Growth Kinetics and Phenol Degradation of Highly Efficient Phenol-degrading Ochrobactrum sp. CH10

作　　者：陈晓华[1,2] 魏刚[1,2] 刘思远[1,2] 孙江曼[1,2] 王芳芳[1,2] 李昊塬[1,2] 刘宇军[1,2] 

机构地区：[1]北京化工大学材料科学与工程学院,北京100029 [2]北京化工大学化工资源有效利用国家重点实验室,北京100029

出　　处：《环境科学》2012年第11期3956-3961,共6页Environmental Science

摘　　要：菌株Ochrobactrum sp. CH10是从北京元大都城垣遗址处的人工湿地筛选到的高效苯酚降解菌.以苯酚为唯一碳源和能源对其进行了生长和苯酚降解特性的研究.该菌生长和降解苯酚的适宜条件为30℃、初始pH 7.0、接种量为5%.在该条件下,初始苯酚浓度为400 mg·L-1,24 h时苯酚完全被降解;初始苯酚浓度为900 mg·L-1时,44 h的降解率为92.3%;初始苯酚浓度为1 000 mg·L-1,48 h时的降解率为82.2%.对该菌株苯酚降解动力学过程进行模拟,符合基质抑制型的Haldane模型,各参数分别为:υmax(最大比降解速率)0.126 h-1,KS(半饱和常数)23.53 mg·L-1,KI(抑制常数)806.1 mg·L-1.该菌在苯酚中的生长动力学符合Andrews模型,表现出与苯酚降解相似的趋势.该菌为目前所发现的Ochrobactrum菌属中苯酚降解能力最强的菌株.该菌株在高效处理含酚废水方面具有广阔的应用前景.The strain Ochrobactrum sp. CH10 was a highly efficient phenol degrading bacterial strain isolated from soil in a constructed wetland in Yuan Dynasty Capital City Wall Relics in Beijing.Growth and biodegradation were investigated in details with phenol as the sole carbon and energy source.The best growth and most efficient phenol biodegradation occurred when the strain was cultured in medium containing 400 mg·L^-1 phenol at initial pH of 7.0 and 30℃,with 5% inoculation volume.The phenol degradation rate was around 100%,92.3 and 82.2% with an initial concentration of 400,900 and 1 000 mg·L^-1 phenol in 24,44 and 48 h,respectively.Phenol degradation kinetic studies indicated that the strain followed Haldane＇s model,and the parameters were： υmax（maximum specific rate）=0.126 h-1,KS（half-saturation constant）=23.53 mg·L^-1 and KI（inhibition constant）=806.1 mg·L^-1.The phenol-limited growth kinetics of CH10 by Andrews＇s model also followed a similar trend to that of phenol degradation.Among all the strains belonging to Ochrobactrum genus,this strain is the most efficient at present.The strain has a good application potential for the phenolic wastewater treatment.

关 键 词：苯酚 生物降解 动力学 OCHROBACTRUM sp. 废水处理 

分 类 号：X172[环境科学与工程—环境科学]