气相色谱法测定液体乳及乳粉中的7种防腐剂  

Study on determination of seven preservatives in milk and milk powder by gas chromatography

作　　者：张红霞[1] 卢克刚[2] 梁秀清[1] 张卉[1] 祝建华[1] 

机构地区：[1]山东省产品质量监督检验研究院,国家加工食品质量监督检验中心(山东),济南250100 [2]山东职业学院,济南250104

出　　处：《中国乳品工业》2012年第10期46-48,共3页China Dairy Industry

摘　　要：应用气相色谱技术建立液体乳及乳粉中山梨酸、苯甲酸、脱氢乙酸、对羟基苯甲酸甲酯、对羟基苯甲酸乙酯、对羟基苯甲酸丙酯和对羟基苯甲酸丁酯共7种防腐剂的定量分析方法。样品酸化后用乙酸乙酯提取，提取液经脱水后直接进样，中等极性毛细色谱柱DB-17分离，氢火焰离子化检测器检测。结果表明，7种防腐剂在质量浓度为10-500mg／L范围内呈良好的线性关系，相关系数在0．999以上，方法的检出限（以信噪比大于3计）为0．2-0．5mg／L，定量限（以信噪比大于10计）为0．7～1．7mg／L，平均加标回收率为96．6％-104．3％，RSD〈5％。该方法前处理简单快速、检测效率高、线性范围宽、灵敏度高、实用性较强。To establish a method for the simultaneous determination of 7 preservatives in milk and milk powder by GC-FID. After acidifica-tion, the sample was extracted with ethyl acetate. The extract was separated using DB-17 middle polarity capillary column and detected by GC with FID detector. The results showed that linear ranges and coefficients of correlation of the method were 10-500 mg/L and above 0.999 for 7 preservatives, respectively. The results indicated that the limits of detection （LODs, S/N〉3） for the 7 preservatives were in the range of 0.2-0.5 mg/L, and the limits of quantitation （LOQs, S/N〉10） were in the range of 0.7-1.7 mg/L.The recoveries were between 96.6% and 104.3% with the relative standard deviations （RSDs） of less than 5%. Conclusion： The developed method is rapid, effective, sensi-tive and practical.

关 键 词：液体乳及乳粉 防腐剂 气相色谱法 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]