单方中药马蹄香和5种微生态制剂中的低聚糖含量测定和比较分析  

Detection and analysis of oligosaccharides in Valeriana Jatamansii Jones and five microecological agents

作　　者：黄永坤[1] 潘媛 肖桦[1] 张峻[1] 刘梅[1] 董坚[1] 刘流[1] 杨成金 王承宇[4] 黄根[4] 

机构地区：[1]昆明医科大学第一附属医院,云南昆明650032 [2]云南省产品质量监督检验研究院,云南昆明650000 [3]云南省白药集团股份有限公司,云南昆明650118 [4]中国军事医学科学院军事兽医研究所,吉林长春130062

出　　处：《中国微生态学杂志》2012年第10期868-872,875,共6页Chinese Journal of Microecology

基　　金：国家科技支撑计划课题(2008BADB4B03)

摘　　要：目的深入认识马蹄香治疗病毒肠炎的有效成分。方法应用戴安CS-3000离子色谱分析仪测定马蹄香散剂和酪酸梭菌二联活菌散剂、双歧杆菌乳杆菌三联活菌片、双歧三联活菌胶囊、布拉酵母菌散剂及乳酸菌素片5种微生态制剂中的10种低聚糖含量。结果低聚糖总含量:双歧三联活菌片>酪酸梭菌二联活菌散剂>双歧三联活菌胶囊>布拉酵母菌散剂>乳酸菌素片>马蹄香散剂。在单个的低聚糖含量中,半乳糖三糖含量:酪酸梭菌二联活菌散剂>双歧三联活菌片>马蹄香散剂>乳酸菌素片>布拉酵母菌散剂>双歧三联活菌胶囊;棉籽糖含量:双歧三联活菌片>双歧三联活菌胶囊>布拉酵母菌散剂>乳酸菌素片>酪酸梭菌二联活菌散剂>马蹄香散剂;水苏糖含量:双歧三联活菌胶囊>布拉酵母菌散剂>乳酸菌素片>酪酸梭菌二联活菌散剂>马蹄香散剂>双歧三联活菌片;毛蕊花糖含量:马蹄香散剂>乳酸菌素片>双歧三联活菌胶囊>双歧三联活菌片>酪酸梭菌二联活菌散剂>布拉酵母菌散剂;潘糖含量:马蹄香散剂>布拉酵母菌散剂>酪酸梭菌二联活菌散剂>其他;鼠李糖含量:乳酸菌素片>布拉酵母菌散剂>马蹄香散剂>其他;麦芽四～七糖含量:乳酸菌素片>马蹄香散剂>布拉酵母菌散剂>其他。结论微生态制剂中低聚糖主要是半乳糖三糖、棉籽糖和水苏糖的含量高。与其他微生态制剂比较,马蹄香散剂中毛蕊花糖和潘糖的含量最高,半乳糖三糖、鼠李糖和麦芽四至七糖的含量也较高,这些低聚糖的含量高可能与改善肠道菌群和治疗病毒性肠炎的有效性有关。Objective To further explore the active ingredients in Valeriana Jatamansii Jones against viral enteritis.Methods The contents of 10 oligosaccharides in Valeriana Jatamansii Jones power,Combined live Clostriudium butyricum bifidobacterium powder,Combined live bifidobacterium lactobacillus tablets,Bifid triple viable capsules,Saccharomyces boulardii sachets and Lacidophilin tablets were determined by DIONEX CS-3000 high performance liquid chromatography-iron analyzer.Results The content of oligosaccharides Combined live bifidobacterium lactobacillus tablets,was the highest,followed by Combined clostriudium butyricum bifidobacterium powder,Bifid triple viable capsules,Saccharomyces boulardii sachets,Lacidophilin tablets,and Valeriana Jatamansii Jones powder.The content of galactose in Combined clostriudium butyricum bifidobacterium powder,was the highest,followed by Combined live bifidobacterium lactobacillus tablets,Valeriana Jatamansii Jones powder,Lacidophilin tablets,Saccharomyces boulardii sachets,and Bifid triple viable capsules.The highest content of raffinose derived from Combined live bifidobacterium lactobacillus tablets,followed by Bifid triple viable capsules,Saccharomyces boulardii sachets,Combined clostriudium butyricum bifidobacterium powder,and Valeriana Jatamansii Jones powder;The content of stachyose in Bifid triple viable capsules was the highest,followed by Saccharomyces boulardii sachets,Lacidophilin tablets,Combined clostriudium butyricum bifidobacterium powder,Valeriana Jatamansii Jones powder,and Combined live bifidobacterium lactobacillus tablets.The hightest content of verbascose came from Valeriana Jatamansii Jones powder,followed by lacidophilin tablets,Bifid triple viable capsules,Combined live bifidobacterium lactobacillus tablets,Combined clostriudium butyricum bifidobacterium powder,and Saccharomyces boulardii sachets.The content of panose in Valeriana Jatamansii Jones powder was the highest,followed by Saccharomyces boulardii sachets,Combined clostriudium butyricum bifidobacterium p

关 键 词：马蹄香 微生态制剂 低聚糖 

分 类 号：S816.7[农业科学—饲料科学]