乳腺癌早期诊断中细针穿刺端粒酶活性检测的临床应用价值  

Clinical application value of telomerase activity by f＇me needle aspiration in the early diagnosis of breast neoplasms

作　　者：李新兵[1] 

机构地区：[1]山东省淄博市中心医院普外科,255000

出　　处：《中国医师进修杂志》2012年第30期9-11,共3页Chinese Journal of Postgraduates of Medicine

摘　　要：目的探讨细针穿刺端粒酶活性检测在乳腺癌早期诊断中的临床应用价值及意义。方法在B超引导下细针穿刺取86例乳腺组织进行端粒酶活性检测及细胞生物学检查，与术后大体标本进行对照分析。结果乳腺癌67例，细针穿刺端粒酶活性检测阳性率为91．0％（61／67）；细针穿刺细胞学检查阳性率为88．1％（59／67）。乳腺良性病变19例，细针穿刺端粒酶活性检测阳性率为26．3％（5／19），细针穿刺细胞学检查阳性率为10．5％（2／19）。细针穿刺端粒酶活性检测特异度为73．7％（14／19），敏感度为91．0％（61／67）；细针穿刺细胞学检查特异度为89．5％（17／19），敏感度为88．1％（59／67）。两者特异度比较差异无统计学意义（P〉0．05），敏感度比较差异有统计学意义（P〈0．05）。对术后留取的乳腺肿物大体标本进行端粒酶活性检测，67例乳腺癌大体标本端粒酶活性检测阳性率为92．5％（62／67），细针穿刺端粒酶活性检测与相应大体标本端粒酶活性检测的符合率为98．4％（61／62）。19例乳腺良性病变大体标本端粒酶活性检测阳性率为10．5％（2／19），细针穿刺端粒酶活性检测与相应大体标本端粒酶活性检测结果比较差异无统计学意义（P〉0．05）。乳腺癌肿瘤直径小、TNM分期高、有局部淋巴结转移者癌组织中端粒酶阳性率较高，差异有统计学意义（P〈0．05）。结论乳腺癌早期诊断中细针穿刺端粒酶活性检测是一种很好的辅助诊断技术，同时对于肿瘤的恶性进展情况也能间接做出一定程度上的评估。Objective To investigate the early diagnostic value and significance of telomerase activity by fine needle aspiration in breast neoplasms tissues, in order to provide basis for clinical application. Methods The tissues of 86 patients which obtained by B-ultrasound guided fine needle aspiration were detected by both telomerase activity and cytologic examination, and compared with general tissue specimen after operation. Results Sixty-seven patients were diagnosed as breast neoplasms after operation. The telomerase activity positive rate by fine needle aspiration was 91.0% （61/67）, the cytologic examination positive rate was 88.1%（59/67）. Benign lesion was 19 patients. The telomerase activity positive rate was 26.3%（ 5/19 ）, the cytologic examination positive rate was 10.5%（2/19 ）. The specificity of telomerase activity was 73.7%（14/19）, the sensitivity was 91.0%（61/67）. The specificity of cytologic examination was 89,5% （17/19）, the sensitivity was 88.1% （59/67）. The specificity between the two methods bad no significant difference （P 〉0.05）,but the sensitivity had significant difference （P 〈0.05）. Postoperative breast neoplasms detection of telomerase activity positive rate was 92.5% （62/67）.The telomerase activity positive rate coincidence of general tissue specimen after operation was 98.4%（61/62）. Nineteen patients was benign mammary gland disease, the telomerase activity positive rate was 10.5% （2/19）. The telomerase activity positive rate had no significance compared with general tissue specimen （P 〉 0.05 ）. The telomerase activity positive rate had significant relationship with neoplasms diameter, TNM classification, partial nodular transfer （P 〈 0.05 ）. Corlelusions Telomerase activity by fine needle aspiration is a good auxiliary diagnosis technique for breast neoplasms. It can also be used to assess the tumor progression indirectly.

关 键 词：乳腺肿瘤 活组织检查 针吸 诊断 端粒酶 

分 类 号：R737.9[医药卫生—肿瘤]