刺五加与其愈伤组织中皂苷合成关键酶基因表达的差异分析  

Analyze on the Expression Differences of Key Genes in Saponins Biosynthesize in Eleutherococcus senticosus and its Callus

作　　者：邢朝斌[1] 吴鹏[1] 龙月红[1] 何闪[1] 梁能松[1] 

机构地区：[1]河北联合大学生命科学学院,河北唐山063000

出　　处：《中药材》2012年第8期1210-1213,共4页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金(30701086);河北省自然科学基金(C2009001252);河北省自然科学基金-石药集团医药联合研究基金(H2012401006)

摘　　要：目的:分析刺五加叶片、叶柄及其愈伤组织中SS、SE和bAS基因表达量的差异。方法:以刺五加叶片和叶柄为外植体,诱导获得愈伤组织。实时荧光定量PCR检测SS、SE和bAS基因的表达量。结果:刺五加SS、SE和bAS基因在刺五加叶片、叶柄及其愈伤组织中都有表达,SS、SE和bAS基因在叶片愈伤组织中的表达量分别为叶片中的89.3%、73.8%和83.4%,在叶柄愈伤组织中的表达量分别为叶柄中表达量的80.2%、87.7%和70.9%。结论:刺五加愈伤组织中SS、SE和bAS基因的表达量显著低于植株中的表达量。Objective： To analyze the expression differences of SS,SE and bAS in leaf,petiole and callus from leaf and petiole of Eleutherococcus senticosus.Methods： Calluses were induced from explants of E.senticosus leaf and petiole.The expression amount of SS,SE and bAS gene was detected by real time PCR.Results：The expression amount of SS,SE and bAS genes in callus from leaf were 89.3%,73.8% and 83.4% of that in leaf,respectively.The expression amount of SS,SE and bAS genes in callus from petiole were 80.2%,87.7% and 70.9% of that in petiole,respectively.Conclusion： The expression amount of SS,SE and bAS gene in callus from E.senticosus are significantly lower than that in plant.

关 键 词：刺五加 愈伤组织 表达 实时荧光定量PCR 

分 类 号：R282.2[医药卫生—中药学]