海马硬化型颞叶癫疒间海马组织中P-gp、p53和凋亡探讨  

Study of P-gp,p53 and apoptosis in the sclerotic hippocampus with mesial temporal lobe epilepsy

作　　者：段宝奇[1] 吴颐[1] 樊练[1] 胡静[1] 朱长乐[2] 高觉民[1] 

机构地区：[1]江苏省中医院神经外科,210029 [2]江苏省中医院病理科,210029

出　　处：《临床神经外科杂志》2012年第5期293-295,共3页Journal of Clinical Neurosurgery

摘　　要：目的探讨海马硬化型颞叶癫疒间患者海马组织中P-gp、p53、凋亡的表达和意义。方法对17例海马硬化型颞叶癫疒间患者的手术标本分别进行免疫组化检查P-gp,p53;原位末端标记检测细胞凋亡;免疫印迹法检测P-gp,p53。结果对照组P-gp主要在血管内皮细胞表达,未见p53阳性细胞,仅有散在凋亡细胞;癫疒间组P-gp分布在血管内皮细胞和胶质细胞,14例有p53阳性细胞,均有凋亡细胞;免疫印迹法P-gp,p53表达较对照组明显增加。结论海马硬化型颞叶癫疒间患者海马组织中有P-gp、凋亡异常表达,p53可能发挥了调节作用。Objective To explore the role of P-gp, p53 and apoptosis in thesclerotichip- pocampus in patients with mesial temporal lobe epilepsy. Methods The specimens were obtained from 17 patients with mesial temporal lobe epilepsy. The expression of P-gp, p53 were measured by immunohistochemistry and Western blot, and the level of apoptosis was determined by TdT-mediated dUTP Nick-End Labeling. Results P-gp and p53 were highly expressed in the sclerotic hippocampus compared with that of control group. Increased apoptosis level of sclerotichippocampus was also observed. Conclusions The expression of P-gp , p53 and the level of apoptosis were significantly increased in the sclerotic hippocampus. P53 may play a role during this procedure.

关 键 词：癫痫 海马硬化 P糖蛋白 凋亡 P53 

分 类 号：R742.1[医药卫生—神经病学与精神病学]