武汉单极虫(粘体门,双壳目)的重描述及基于18S rDNA序列系统发育分析  

REDESCRIPTION OF THELOHANELLUS WUHANENSIS XIAO ET CHEN (MYXOZOA, MYXOSPOREA) INFECTING ALLOGYNOGENETIC CRUCIAN CARP (CARASSIUS AURATUS GIBELIO) AND PHYLOGENIC ANALYSIS BASED ON 18S RDNA SEQUENCE

作　　者：竹攸汀[1] 陆宏达[1] 蔡水局[1] 

机构地区：[1]上海海洋大学水产与生命学院,农业部淡水水产种质资源重点实验室,上海201306

出　　处：《动物分类学报》2012年第4期681-686,共6页Acta Zootaxonomica Sinica

基　　金：上海市重点学科建设项目(Y1101)资助~~

摘　　要：通过光镜、扫描电镜和18SrDNA序列对比,对寄生于异育银鲫Carassius auratus gibelio体表的粘孢子虫进行了形态学和分子生物学研究,鉴定其为武汉单极虫Thelohanellus wuhanensis,并发现了形态学新特征:膜状鞘的起始位置不同,且仅包围孢子后部;壳瓣底部内侧具"V"形褶皱;一片壳瓣前端有突起结构;缝脊直,突出明显;测得极丝全长为158.5～179.2(170.7±5.7)μm。经18S rDNA序列对比,与T. wuhanensis[HQ613410]的相似率为99.87%。系统发育分析表明,单极虫与碘泡虫具有非常近的亲缘关系,将单极虫属与碘泡虫属二者归属于碘泡科的分类方法更合理。Thelohanellus wuhanensis Xiao et Chen, 1993 infecting skin and fan of fry of Allogynogenetic crucian carp （ Carassius auratus gibelio ） was isolated. To augment the original description, T. wuhanensis is redescribed here by morphological and molecular biological data. Mature spores are melon seed, 21.9 - 26. 9 （ 24.6±1.0） μm long and 11. 4-15.5 （13.7±1.0） μm wide in frontal view and spindle shape, 10. 8 - 14. 1 （ 11.8 ± 0. 5 ） μm thick in lateral view. Polar capsules are approximately spherical, 9.6 - 12.8 （ 11.5 ±0.7 ） μm long and 8.1 - 10. 3 （9. 1 ± 0. 5 ） μm wide, containing polar filaments coil 7 - 10 turns. Some new characteristics of T. wuhanensis are found. Mucous envelopes only surround posterior part of spores. Vertical distance from front end of spores to mucous envelope is 5.0 - 16.7 （ 10. 4 ± 2.4 ）. ＂V＂ - shaped edges marking on the spores＇ shells. The spore have asymmetric front end, one of which shell has a protuberance on the front end. Sutural ridges are straight. Total length of polar filaments are measured.Phylogenetic analysis indicates that Thelohanellus and Myxobolus have a very close genetic relationship. It is reasonable that both THelohanellus and Myxobolus belong to the family Myxobolidae.

关 键 词：武汉单极虫 光镜 扫描电镜 形态学 18S RDNA 

分 类 号：S941.5[农业科学—水产养殖] Q959[农业科学—水产科学]