FasL反义寡核苷酸对舌鳞癌细胞Tca8113增殖和凋亡的影响  

Effect of FasL antisense oligonucleotide on proliferation and apoptosis of Tca8113

作　　者：方丽[1] 陈乔尔[1] 任翠平[2] 沈际佳[2] 

机构地区：[1]安徽医科大学口腔医学院,安徽医科大学附属口腔医院,安徽省口腔临床医学重点学科,安徽省口腔疾病研究省级实验室,中央与地方共建口腔医学中心实验室,合肥230032 [2]安徽医科大学病原生物学教研室,合肥230032

出　　处：《安徽医科大学学报》2012年第11期1300-1303,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省自然科学基金(编号:11040606M175)

摘　　要：目的探讨Fas配体(FasL)反义寡核苷酸(ASO)对舌鳞癌细胞系Tca8113细胞增殖和凋亡的影响。方法设计合成特异性的FasL ASO,并用阳离子脂质体介导转染Tca8113细胞。荧光倒置显微镜观察及流式细胞术(FCM)检测转然前后的转染效率,四甲基偶氮唑蓝(MTT)法检测不同浓度ASO在转染后不同时间对Tca8113细胞增殖的影响;FCM测定转染前后诱导Tca8113细胞的凋亡情况。结果通过荧光倒置显微镜观察及FCM检测发现在脂质体的量一定的情况下,在一定范围内,ASO的浓度越高,转染效率越高。MTT结果显示ASO对Tca8113细胞的增殖有明显抑制作用(P<0.05),FCM对细胞凋亡的检测结果显示反义链组与正义链组、空白对照组及脂质体组相比,总凋亡率有明显差异(P<0.05)。结论 FasL ASO对Tca8113细胞的增殖有一定的抑制作用,能够促进其凋亡,因此FasL ASO有望成为舌鳞癌的潜在的基因治疗方法。Objective To investigate the effect of Fas ligand（FasL） antisense oligonucleotide（ASO） on prolifera- tion and apoptosis of TcaS113 cells in vitro. Methods Specific phosphorothioate ASO were synthesized and trans- fected into TcaS113 cells with lipofectamineTM2000. The growth and the transfection of Tca8113 cells were observed by inverted microscope and the transfection efficiency was tested by flow cytometry （FCM）. The cell viability of Tca8113 cells was assessed by methyl thiazolyl tetrazolium （MTT） assay and the apoptosis rate was detected by FCM before- and after-transfection. Results The FasL ASO inhibited cell proliferation in a dose- and time-depend- ent manner within limits. The total apoptosis rate was apparently increased （P 〈 0. 05 ） in antisense group compared with the control group, the lipofectamine group and the sense group. Conclusion FasL ASO can suppress prolifera- tion and induce significant apoptosis of Tca8113 cells. FasL ASO may become a sort of gene therapy of tongue squamous cell carcinoma.

关 键 词：FAS FAS配体 反义寡核苷酸技术 舌鳞癌细胞 

分 类 号：R730.5[医药卫生—肿瘤] R739.8[医药卫生—临床医学]