TMPRSS2-ERG、AMACR和p63在前列腺癌中的表达及相关性  

Expression of TMPRSS2-GER,AMACR and p63 in prostate cacinoma and their correlation

作　　者：孙文国[1] 夏利 蒋雷鸣[1] 杨伟娇[3] 莫文发 

机构地区：[1]桂林医学院附属医院 泌尿外科 [2]皮肤性病科 [3]中山大学附属第三医院泌尿外科,广州510000 [4]病理科,桂林541001

出　　处：《安徽医科大学学报》2012年第11期1351-1355,共5页Acta Universitatis Medicinalis Anhui

基　　金：广西桂林市科学研究与技术开发计划(编号:20120121-1-2)

摘　　要：目的分析跨膜丝氨酸蛋白酶2(TMPRSS2)-ERG、甲酰基辅酶A-旋酶(AMACR)、基底细胞标记抗体(p63)在不同前列腺组织中的表达情况,并探讨其与前列腺癌临床病理特征之间的相关性。方法分别用快捷免疫组织化学染色法和荧光原位杂交(FISH)法检测50例前列腺癌(PCa)、30例前列腺增生(BPH)、10例高级别前列腺上皮内肿瘤(HG-PIN)和10例前列腺转移癌患者组织中的AMACR、p63蛋白和TMPRSS2-ERG融合基因的表达情况,并分析其相关性。结果 TMPRSS2-ERG、AMACR在PCa中表达阳性率显著高于良性BPH对照组(P<0.01);p63在PCa和前列腺转移癌中表达阳性率显著低于BPH和HGPIN(P<0.01)。在PCa中,TMPRSS2-ERG融合基因稳定高表达,不受血清PSA大小、Gleason评分高低及TNM分期的影响,而AMACR表达与血清PSA大小、Gleason评分高低及TNM分期密切相关(P<0.05);TMPRSS2-ERG、AMACR、p63在PCa中的表达具有相关性(P<0.05);TMPRSS2-ERG和AMACR的阳性符合率随AMACR信号增强而增强,+、++、+++分别对应为0、66.7%(4/6)、83.3%(30/36),呈明显的正相关(rs=0.390,P=0.005),阴性符合率为71.4%(5/7)。结论在PCa诊断中TMPRSS2-ERG融合基因可作为独立检测因子,TM-PRSS2-ERG和AMACR在PCa诊断中具有高符合率,TM-PRSS2-ERG、AMACR和p63在PCa的疾病发生、发展中具有某种相互作用。联合检测TMPRSS2-ERG、AMACR、p63可以在基因和蛋白两个层面对PCa做出更加稳定可靠的早期诊断。Objective To investigate the expression of TMPRSS2-ERG, AMACR and p63 in prostate and their cor-relation with clinicopathological factors. Methods Immunohistochemistry was used to detect the expression of AM- ACR and p63 and fluorescence in situ hybridization（FISH） was used to detect TMPRSS2-ERG gene fusion in 50 cases of prostate carcinoma, 30 cases of benign prostatic hyperplasia, 10 cases of high grade prostatic intraepithelial neoplasia（HGPIN） and 10 cases of prostate metastatic cancer tissue, then their correlation was analyzed. Results The expression of TMPRSS2-ERG gene fusion and AMACR was higher in prostate carcinoma but lower in benign prostatic hyperplasia tissues（P 〈0. 01 ）. And p63 was lower in prostate carcinoma and prostate metastatic cancer than benign prostatic hyperplasia and high grade prostatic intraepithelial neoplasia （ P 〈 0. 01 ）. TMPRSS2-ERG gene fusion was stably highly expressed in prostate carcinoma, and the expression was not related with the degree of prostate-specific antigen, Gleason score and TNM, however, p63 and AMACR were highly related （P 〈 0.05 ）. Statistical correlation was found between the expression of TMPRSS2-ERG and AMACR （ P 〈 0. 05 ）. And the coin- cidence rate was enhanced with the increase of signal in AMACR, and the negative coincidence rate was 71.4%. Conclusion In the diagnosis of prostate cancer, TMPRSS2-ERG gene fusion is considered to be an independent detection factor;TMPRSS2-ERG and AMACR have a high coincidence rate. TMPRSS2-ERG, AMACR and p63 in prostate carcinoma progression may have a synergistic effect. Joint detection of TMPRSS2-ERG, AMACR and p63 can make a more reliably early diagnosis of prostate cancer in the levels of gene and protein.

关 键 词：前列腺癌 免疫组织化学染色法 荧光原位杂交 TMPRSS2-ERG融合基因 AMACR P63 

分 类 号：R737.2[医药卫生—肿瘤]