树鼩血栓性脑缺血磁共振成像研究  

The Reaserch of Magnetic Resonance Imaging of Thrombotic Cerebral Ischemia in Tree Shrews

作　　者：何波[1] 韩丹[1] 何亮[2] 张峰睿[1] 李树清[2] 

机构地区：[1]昆明医科大学第一附属医院医学影像中心,650032 [2]昆明医科大学病理生理教研室,650500

出　　处：《临床放射学杂志》2012年第10期1492-1496,共5页Journal of Clinical Radiology

基　　金：国家自然科学基金面上资助项目(编号:30971171);云南省教育厅科研资助项目(编号:2010Y176);昆明医科大学博士研究生创新基金资助项目(编号:2009D04)

摘　　要：目的研究树鼩血栓性脑缺血动物模型的MRI表现,探讨其动态演变规律。方法采用3.0 T MR在缺血后4 h、24 h、48 h及72 h对树鼩脑部行MRI,观察各时间点MRI表现,测量和计算出各时间点扩散加权成像(DWI)、T2WI异常信号体积;分别应用HE染色、红四氮唑(2,3,5-triphenyltetrazolium chloride,TTC)染色技术显示脑缺血后不同时间点病理改变和皮层梗死体积的变化。结果缺血后4 h MRI可见信号改变,T2WI为高信号,T1WI为低信号,DWI为高信号,增强扫描可见强化;TTC染色所测体积与MRI体积比较,4 h时DWI及T2WI所测体积大于TTC(P<0.05),病理显示周边为水肿带;缺血后4 h至24 h梗死灶体积增大明显(P<0.05);光镜示缺血后中心区神经元可见皱缩,脱失明显,出现嗜酸神经元。结论 MRI可动态反映树鼩血栓性脑缺血动物模型缺血后不同时间点的病理演变过程。Objective To study the dynamic evolution of magnetic resonance imaging of thrombotic cerebral ischemia in Tree Shrews.Methods The tree shrew were scaned by 3.0 T MRI in 4 h,24 h,48 h and 72 h after ischemia.The MR findings,pathological features and TTC staining were observed.The ischemic volume in DWI,T2WI and TTC were measured and calculated in each time point.Results The signal changes could be seen on MRI in 4 h after ischemia.The ischemic lesion showed high sigal on T2WI,low signal on T1WI and high signal on DWI.The ischemic volume measured by MRI were greater than TTC staining in 4h after ischemia（P〈0.05）.The pathology revealed peripheral edema in 4 h after ischemia.The ischemic volume increased significantly from 4 h to 24 h after ischemia（P〈0.05）.The pyknosis,loss and eosinophilic changes of the neurons could be detected in pathology.Conclusion MRI can reflect dynamically the pathological changes of thrombotic cerebral ischemia in Tree Shrews.

关 键 词：树鼩 光化学 脑缺血 磁共振成像 

分 类 号：R743[医药卫生—神经病学与精神病学]