Satb2和Cbfα1基因在人牙龈成纤维细胞中的表达  

Expression of Satb2 and Cbfα1 gene in human gingival fibroblast cells

作　　者：吕春旭[1] 陈晓霞[1] 张瑾[1] 孙钦峰[1] 

机构地区：[1]山东大学口腔医院牙周科山东省口腔生物医学重点实验室,山东济南250012

出　　处：《牙体牙髓牙周病学杂志》2012年第10期561-564,共4页Chinese Journal of Conservative Dentistry

基　　金：山东省科技攻关课题(2009GG10002052)

摘　　要：目的:观察Satb2和Cbfα1在人牙龈成纤维细胞(HGFs)中的表达,为进一步研究二者在牙周组织再生中的作用奠定基础。方法:体外组织块法培养HGFs,并观察其形态和生长方式;同时采用RT-PCR和Western blot方法检测HGFs中Satb2和Cbfα1的表达。结果:体外培养的HGFs中hSatb2和hCbfα1 mRNA均呈阳性表达,但在蛋白质水平只检测到hSatb2蛋白的表达。结论:Satb2和Cbfα1在HGFs中有不同程度的表达,但能否作为刺激HGFs骨向分化的有效生长因子尚需进一步研究。AIM： To investigate the expression of Satb2 and Cbfαl gene in human gingival fibroblast cells （HGFs）. METHODS： Tissue block method was useded to separate and cultivate HGFs, cell morphology and cell growth were observed. RT-PCR and Western blot were used to study the expression of the Satb2 and Cbfαl gene. RESULTS： Human gingival fibroblast cells were expanded in vitro. The expression of Satb2 mRNA and protein could be detected in normal HGFs, but for Cbfα, only mRNA expression was detected in HGFs. CONCLURION： The differential expression of Satb2 and CbfalmRNA and protein suggests that these genes may play a role at varying degrees. Further study is required to determine whether they are effective growth factors during the osteogenic differentiation of the cells.

关 键 词：Satb2 Cbfα1 人牙龈成纤维细胞 

分 类 号：R780.2[医药卫生—口腔医学]