DNA甲基转移酶3b在人胰腺癌组织中的表达及其意义  

Expression and role of DNMT3b in human pancreatic cancer tissue

作　　者：徐岷[1] 姚志新[1] 朱立宁[1] 徐萍[1] 周志华[2] 龚燕芳[3] 满晓华[3] 吴莺[1] 张尤历[1] 

机构地区：[1]江苏大学附属医院消化科,江苏镇江212001 [2]解放军第101医院病理科 [3]第二军医大学长海医院消化科

出　　处：《中华胰腺病杂志》2012年第5期323-325,共3页Chinese Journal of Pancreatology

基　　金：江苏省自然科学基金（BK2009210）;江苏省卫生厅科研项目（H200836）;镇江市科研计划项目（SH2009012）

摘　　要：目的检测人胰腺癌组织中DNA甲基化转移酶3b（DNMT3b）的表达，分析其与肿瘤临床病理特征的相关性。方法应用蛋白质印迹法检测12例配对人胰腺癌和癌旁组织中DNMT3b的表达；免疫组织化学法检测59例胰腺癌组织中DNMT3b的表达，并分析其与肿瘤临床病理特征的相关性。结果蛋白质印迹法结果显示，胰腺癌及癌旁组织DNMT3b蛋白表达量分别为0．69±0．13和0．14±0．03，癌组织的表达量明显高于癌旁组织（t=4．464，P〈0．05）。免疫组化结果显示，胰腺癌组织DNMT3b阳性高表达率为59％，癌旁组织阴性表达。肿瘤组织DNMT3b表达强度与TNM分期和淋巴结转移相关。结论DNMT3b在胰腺癌组织中高表达，其表达与肿瘤的恶性生物行为有关。Objective To investigate the expression of DNMT3b and its correlation with clinicopathological parameters of pancreatic cancer. Methods The expressions of DNMT3b protein in 12 pancreatic cancer tissues and para-cancerous tissues were detected by Western blot. The expressions of DNMT3b protein in 59 pancreatic cancer tissues were detected by immunohistochemistry. The association of DNMT3b expression and clinicopathological parameters of pancreatic carcinoma were analyzed. Results Western blot results showed the expressions of DNMT3b protein in pancreatic cancer tissues and para-cancerous tissues were 0.69 ± 0.13and 0.14± 0.03, and the protein level of DNMT3b in pancreatic cancer tissues were significantly higher than that in para-cancerous tissues （t = 4. 464, P 〈0.05 ）. Immunohistochemistry examination showed that the positive rates of DNMT3b protein were 59% in pancreatic cancer tissues and negative in para-cancerous tissues. DNMT3b expression was positively correlated with the TNM stage and lymph node metastasis. Conclusions DNMT3b is highly expressed in pancreatic cancer tissues, and it is related with malignant biological behaviors of oancreatic cancer cells.

关 键 词：胰腺肿瘤 甲基化 DNA甲基转移酶3B 

分 类 号：R735.9[医药卫生—肿瘤]