三种胰腺癌细胞株Mesothelin的表达及成瘤速度的比较  

Comparison of expression of mesothelin among three kinds of pancreatic cancer cell lines anddevelopment speed in their nude mouse models

作　　者：潘璜[1] 吴洪玉[2] 陈士跃[1] 刘敬禹[1] 邵成伟[1] 田建明[1] 

机构地区：[1]解放军庐山疗养院放射科,江西九江332000 [2]第二军医大学长海医院消化内科

出　　处：《中华胰腺病杂志》2012年第5期326-328,共3页Chinese Journal of Pancreatology

基　　金：基金项目：国家自然科学基金（30970801）

摘　　要：目的比较3种人胰腺癌细胞株在体外和裸鼠体内Mesothelin的表达及裸鼠皮下种植瘤生长速度的差异。方法培养人胰腺癌细胞株SW1990、BxPC3、PANC1，取对数生长期分别注射于裸鼠左腋窝皮下，每周测量裸鼠皮下种植瘤的长、短径，计算体积，SW1990、BxPC3组裸鼠观察3周，PANC1组裸鼠观察5周；用蛋白质印迹法分别检测3种细胞及裸鼠皮下种植瘤组织中的Mesothelin表达，用免疫组化染色检测3种裸鼠皮下种植瘤组织中Mesothelin表达。结果人胰腺癌细胞株体外Mesothelin表达的强弱顺序是BxPC3〉PANC1〉SW1990，体内种植瘤组织表达的强弱顺序是SW1990〉BxPC2〉PANC1。3种人胰腺癌细胞种植于裸鼠皮下的成瘤率均为100％，各组肿瘤的生长速度顺序为SW1990〉BxPC3〉PANC1。结论不同人胰腺癌细胞株Mesothelin的表达量不同，裸鼠皮下种植瘤的生长速度亦不同，两者无相关性。Objective To compare the mesothelin expressions in 3 human pancreatic cancer cell lines between in vitro and in vivo and the developing speed among the subcutaneous tumors implanted with the 3 human pancreatic cancer cell lines in nude mice. Methods The human pancreatic cancer cell lines （ SW1990, BxPC3 and PANC1 ） were cultured and then were implanted subcutaneously into left axillas of nude mice. The volumes of these subcutaneous tumors were recorded every week to estimate their developing speed. The mice implanted with SW1990 and BxPC3 cells were observed for three weeks, while the mice implanted with PANC1 cell were observed for five weeks. The Western blot method was used to measure the expressions of mesothelin in the 3 kinds of cells and subcutaneous tumors, while immunohistochemical staining was applied to determine the expressions of mesothelin in 3 kinds of subcutaneous tumors. Results The sequence of quantities of expressions of mesothelin in these cell lines in vitro were BxPC3 〉 PANC1 〉 SW1990, and the sequence of quantities of expressions in vivo were SW1990 〉 BxPC3 〉 PANC1. One handrued percent of the tumors grew out successfully, and the sequence of speeds of their growth was SW1990 〉 BxPC3 〉 PANC1. Conclusions The mesothelin expressions among 3 kinds of pancreatic cancer cell line are different. The developing speeds of tumors originated from different subcutaneous tumors in nude mice are also different, andthere is no associalion between them.

关 键 词：胰腺肿瘤 细胞系 肿瘤 Mesothelin蛋白 小鼠 裸 印迹法 蛋白质 免疫组 织化学 

分 类 号：R735.9[医药卫生—肿瘤]