VEGF在头颈部Kaposi肉瘤中的表达及其与HHV-8病毒相关性研究  

Expression of VEGF in Kaposi's Sarcoma of Head and Neck and its Correlation with HHV-8

作　　者：郑军[1] 黎昌学[1] 尹宏斌[2] 徐江[1] 

机构地区：[1]石河子大学医学院第一附属医院口腔科,新疆石河子832008 [2]新疆医科大学第一附属医院口腔颌面外科

出　　处：《口腔医学研究》2012年第10期1056-1058,共3页Journal of Oral Science Research

摘　　要：目的:研究血管内皮生长因子(vascular endothelial growth factor,VEGF)在头颈部Kaposi肉瘤(Kaposi’ssarcoma,KS)中的表达,并分析人类8型疱疹病毒(Human Herpesivirus-8,HHV-8)感染对其表达的影响,探讨VEGF与头颈部KS发生发展的关系。方法:应用逆转录聚合酶链反应(RT-PCR)检测3例头颈部KS和3例正常对照组织中VEGF mRNA表达,免疫组化S-P法检测9例头颈部KS和正常对照组织中VEGF蛋白的表达,并用Western blotting检测感染有HHV-8病毒的BCBL-1细胞和未感染HHV-8病毒的BJAB细胞中VEGF蛋白的表达水平。结果:VEGF在头颈部KS中的mRNA和蛋白表达水平均高于正常对照组织(P<0.05),且VEGF蛋白在BCBL-1细胞中的表达较BJAB细胞高,差异有显著性(P<0.01)。结论:VEGF在头颈部Kaposi肉瘤中高表达,并且其高表达与感染HHV-8密切相关。Objective： To study the expression of VEGF in KS of head and neck,and analyze the effect of HHV-8 infection on the VEGF expression,so as to investigate its role in the pathogenesis of KS of head and neck.Methods： Reverse transcription-polymerase chain reaction（RT-PCR） was used to detecte the VEGF mRNA expression in 3 cases with KS of head and neck and 3 cases with tumor-free margins as control.S-P immunohistochemical method was used to detecte the VEGF protein expression in 9 cases with KS of head and neck and 9 cases with tumor-free margins as control.Western blotting was used to detecte the expression of VEGF in BCBL-1（HHV-8＋） and BJAB（HHV-8-） cells.Results： The higher mRNA and protein expression level of VEGF were observed in KS of head and neck than in normal tissues（P0.05）;and the expression of VEGF in BCBL-1 cells was higher than that in BJAB cells（P0.01）.Conclusion： VEGF is overexpressed in KS of head and neck,and the overexpression is closely correlated with HHV-8 infection.

关 键 词：血管内皮生长因子 KAPOSI肉瘤 人类8型疱疹病毒 

分 类 号：R739.91[医药卫生—肿瘤]