鼠MDM-2 SNP309位点与肝细胞癌发病年龄与风险关系的分析  

Association of MDM-2 SNP309 polymorphism and age at onset and risk of hepatocellular carcinoma

作　　者：张艳艳[1] 毛良勤[1] 曾小云[1] 谢志春[1] 仇小强[2] 余红平[1] 

机构地区：[1]广西医科大学公共卫生学院流行病学教研室,广西南宁530021 [2]桂林医学院公共卫生学院流行病学教研室,广西桂林541004

出　　处：《中华肿瘤防治杂志》2012年第17期1285-1289,共5页Chinese Journal of Cancer Prevention and Treatment

基　　金：国家自然科学基金(30660162);广西科学研究与技术开发项目(0719006-2-13);广西科学基金(0991126);广西大型仪器协作网测试补助(668-2008-081)

摘　　要：目的:探讨广西地区人群鼠双微粒体-2基因(MDM-2)启动子区309位点单核苷酸多态性(SNP)与肝细胞癌(hepatocellular carcinoma,HCC)发病年龄和发病风险的关系。方法:运用聚合酶链反应-限制性片段长度多态性方法,对985例HCC病例和992例非肿瘤对照者的MDM-2SNP309位点(T>G,rs2279744)基因型进行检测,并分析该SNP与HCC发病年龄和发病风险的关系。结果:经年龄、性别、民族、吸烟、饮酒、HBV及HCV感染等因素校正后,MDM-2SNP309位点与HCC发病风险之间无统计学关联(TG vs TT:OR=1.19,95%CI:0.86～1.65;GG vs TT:OR=1.28,95%CI:0.89～1.85;TG+GGvs TT:OR=1.22,95%CI:0.90～1.66)。在女性HCC患者中,与携带MDM-2SNP309位点TG+GG基因型的女性HCC患者相比(44.8岁),携带TT基因型的女性患者HCC发病年龄提前4.6岁(49.4岁),Log-rank检验:χ2=7.372,P=0.007。在男性患者中未发现此类似结果。结论:MDM-2SNP309位点多态性可能对HCC的发病风险无单独效应作用,但其TT基因型可能与女性HCC的发病年龄提前有关联。本研究结果需要大样本量的研究进一步验证。OBJECTIVE： To explore associations between MDM-2 single nucleotide polymorphism （SNP） 309 （T G, rs2279744） and the age at onset and risk of hepatocellular carcinoma （HCC） in Guangxi population. METHODS： A hospital-based case-control study was conducted in 985 cases with HCC and 992 cancer-free controls. SNP309 genotypes were detected using a polymerase chain reaction （PCR）-restriction fragment length polymorphism （RFLP） assay, and as- sociations of MDM-2 SNP309 with risk and age of onset of HCC were assessed. RESULTS： The variant genotypes of MDM-2 SNP309 were not significantly associated with risk of HCC （TG vs TT： OR=I. 19, 95%CI： 0. 86--1. 65 ; GG vs TT： OR=1.28, 95%CI： 0.89--1.85; TG＋GGvsTT： OR=1.22, 95%CI： 0.90--1.66）. It was found that female patients carrying the TT genotype （44.8 year-old） showed a 4.6-year earlier age at onset, compared with those carrying the TG＋GG genotypes （49.4 year-old）,X2 =7. 372, P=0. 007. Similar observation was not seen among male. CONCLU- SIONS.. The findings suggest that MDM-2 SNP309 may not have the independent effect on the susceptibility to HCC. The MDM-2 SNP309 TT genotype may be associated with an earlier age at onset of HCC in women. Further studies with large sample are warranted to validate these results.

关 键 词：癌 肝细胞 基因 MDM-2 多态性 单核苷酸 发病年龄 肝肿瘤/流行病学 

分 类 号：R735.7[医药卫生—肿瘤]