不同浓度吗啡与5-氟尿嘧啶联合应用对MCF-7乳腺癌细胞Bcl-2、Bax表达的影响  

Effects of different concentrations of morphine combined with 5-fluouracil on the expressions of Bcl-2 and Bax in MCF-7 breast cancer cells

作　　者：王志学[1] 孙立新[2] 李汝泓[1] 雷燕[3] 闫彩云[1] 郝振军 

机构地区：[1]承德医学院附属医院麻醉科,067000 [2]承德医学院附属医院中心实验室,067000 [3]承德医学院附属医院手术室,067000 [4]承德市妇幼保健院麻醉科,067000

出　　处：《实用医学杂志》2012年第20期3441-3443,共3页The Journal of Practical Medicine

基　　金：承德市科学技术研究与发展计划项目(编号:201121037)

摘　　要：目的:研究吗啡与5-氟尿嘧啶(5-Fu)联合应用对MCF-7乳腺癌细胞Bcl-2、Bax表达的影响。方法:药物作用48h后通过Western Blot检测Bcl-2、Bax表达情况。结果:Bcl-2表达情况:未处理组最强。单独应用吗啡时,250μmol/L及1250μmol/L引起Bcl-2表达减弱。单独应用500μmol/L5-Fu时Bcl-2表达减弱。与5-Fu联合应用,随其中吗啡浓度的增加,Bcl-2表达逐渐减弱,且均具有协同作用。Bax表达情况:未处理组最弱。单独应用吗啡时,250μmol/L及1250μmol/L引起Bax表达增强。单独应用500μmol/L5-Fu时Bax表达增强。与5-Fu联合应用,随着吗啡浓度的增加,Bax表达逐渐增强,且均具有协同作用。结论:一定浓度的吗啡、5-Fu及联合应用于MCF-7乳腺癌细胞48h能下调Bcl-2表达、上调Bax表达,且与其中吗啡的浓度有一定的剂量依赖关系,两药联合应用具有协同作用。Objective To investigate the effects of morphine combined with 5-fluouracil（5-Fu） on the expressions of Bcl-2 and Bax in MCF-7 breast cancer cells.Methods After being treated by morphine combined with 5-Fu for 48 h,the expressions of Bcl-2 and Bax in MCF-7 breast cancer cells were detected by Western Blot.Results The expression of Bcl-2 was the strongest in the untreated group,and was decreased by 250 μmol/L and 1 250 μmol/L of morphine and 500 μmol/L of 5-Fu,and was decreased along with the increasing of morphine by the combination of morphine and 5-Fu.The expression of Bax was the weakest in the untreated group,and was increased by 250 μmol/L and 1 250 μmol/L of morphine and 500 μmol/L of 5-Fu,and was increased along with the increasing of morphine by the combination of morphine and 5-Fu.The synergisms were found in all the 3 groups.Conclusions Morphine in certain concentration,5-Fu and the combination of the two had the down-regulating effects on Bcl-2 expression and up-regulating effects on Bax expression in MCF-7 breast cancer cells,and in a certain dose dependent manner.Synergisms were shown in the combination of morphine and 5-Fu.

关 键 词：吗啡 5-氟尿嘧啶 MCF-7 BCL-2 BAX 

分 类 号：R737.9[医药卫生—肿瘤]