原花青素对肾血管性高血压大鼠血压及血清抗氧化能力的影响  

Effects of procyanidin on blood pressure and serum antioxidant ability in renovascular hypertensive rats

作　　者：卢律达 黄义 郭晓兰 王以武[2] 

机构地区：[1]湘潭市中心医院,湖南湘潭411100 [2]重庆医科大学药学院,重庆400016

出　　处：《中国医院药学杂志》2012年第20期1631-1633,1639,共4页Chinese Journal of Hospital Pharmacy

摘　　要：目的:探讨原花青素对肾血管性高血压大鼠血压及血清抗氧化能力的影响。方法:采用"两肾一夹"(2K1C)法建立大鼠肾血管性高血压病模型,术后2周,选择40只尾动脉血压升高超过130 mmHg的大鼠按照随机数字表法分为4组,每组10只,分别为:空白对照组高血压模型组、原花青素低剂量治疗组、原花青素高剂量治疗组及氯沙坦组。在对大鼠应用药物治疗后的第4、5、6周,测定2组大鼠的尾动脉血压、血清中超氧化物歧化酶(SOD)活性、一氧化氮(NO)以及丙二醛(MDA)的含量,同时测定总抗氧化能力(T-AOC);应用Western-blot方法检测大鼠腹主动脉中的内皮素-1(ET-1)蛋白表达。结果:治疗4周后,原花青素高、低剂量能显著降低肾血管性高血压大鼠的尾动脉血压(P<0.01,P<0.05),且降压作用维持至第6周;与模型组相比较,原花青素高、低剂量治疗组使肾血管性高血压大鼠血清中MDA含量、ET-1表达值、T-AOC显著降低(P<0.01,P<0.05),使肾血管性高血压大鼠血清中NO含量与SOD活性显著增高(P<0.01,P<0.05)。结论:原青花素能够显著降低肾血管性高血压大鼠尾动脉收缩压,同时对大鼠的血清具有很强的抗氧化能力,主要的作用机制可能与其增加一氧化氮的释放及产生以及降低ET-1的蛋白在血管中的表达相关。OBJECTIVE To investigate the effects of procyanidin on blood pressure and serum antioxidant ability in renovas cular hypertensive（RH）rats. METHODS The RH rat model was established by 2KIC method. Two weeks after operation,50 rats were selected according to the increased tail systolic pressure above 130 mmHg and randomly divided into 3 groups（n = 10）：blank control group, renovascular hypertensive model group, procyanidin high dose treated group , procyanidin low dose treated group and losartan group. The tail systolic pressure, the activity of superoxide dismutase （SOD） , nitric oxide （NO） lev el and the content of malonaldehyde （MDA） in serum and total antioxidant capacity （T-AOC） were determined on the 4th,5th, 6th week after treatment. Western blott was performed to detect the expression of endothlin-1 （ET-1） in abdominal aortic tissues. RESULTS The procyanidin high and low dose could dramatically dramatically reduce the tail systolic pressure in renovascular hypertensive rats 4 weeks after treatment compared to betore treatment（P〈0.01 ,P〈0.05）and the effect contmued to the 6th week;The procyanidin high and low dose could dramatically reduce the content of MDA, expression of ET-1, T-A0C and in crease the NO level,activity of SOD compared to model group（P〈0. 01 ,P〈0.05）. CONCLUSION Procyanidin significantly decreases the tail systolic pressure in RH rats by increasing antioxidative ability and NO production, and reducing the expression of ET-1 in aortic endothelium of the rats.

关 键 词：原花青素 肾血管性高血压 大鼠 抗氧化能力 

分 类 号：R285.5[医药卫生—中药学]