腹腔冲洗液BNIP3基因甲基化与胃癌患者腹膜微转移及疾病的相关性研究  

Correlation Study between Gastric Cancer Peritoneal Micrometastases and BNIP3 Methylation in Peritoneal Lavage Fluid

作　　者：王银玲[1] 王亿龙[2] 王齐晖[1] 李花[1] 周立平[1] 李富顺[1] 

机构地区：[1]中国医科大学附属第一医院检验科,沈阳110001 [2]中国医科大学附属第一医院肿瘤放射治疗科,沈阳110001

出　　处：《中国医科大学学报》2012年第10期934-936,共3页Journal of China Medical University

基　　金：辽宁省教育厅高校科研计划(L2010695)

摘　　要：目的探讨胃癌术中腹腔冲洗液游离细胞BNIP3基因启动子区甲基化水平与胃癌腹膜微转移及疾病进展的相关性。方法收集80例胃癌患者的胃癌组织与相应癌旁组织及术中腹腔冲洗液。用甲基化特异的聚合酶链式反应(MSP)方法检测BNIP3基因启动子区异常高甲基化。结果胃癌组织的BNIP3甲基化阳性率显著高于癌旁组织;腹腔冲洗液游离细胞BNIP3甲基化阳性率在胃癌PTNM分期、淋巴结转移,肿瘤浸润深度间比较,差异均有统计学意义(P<0.05)。结论应用MSP法检测腹腔冲洗液游离细胞BNIP3基因异常甲基化可用于预测胃癌患者腹膜微转移及评估疾病进展。Objective To investigate whether gene methylation of BNIP3 in the peritoneal fluid（PF） predicts peritoneal micrometastases in gastric cancer patients.Methods Eighty pairs of adjacent normal gastric mucosa and corresponding primary cancer tissues were collected from surgery,and peritoneal lavage fluid was also obtained from these 80 patients.The gene methylation of BNIP3 were analyzed by quantitative methylation-specific polymerase chain reaction（MSP）.Results Gene BNIP3 showed a significantly higher methylation level in primary cancer tissues than the adjacent normal gastric mucosa（P 0.05）.Conclusion BNIP3 methylation in peritoneal lavage fluid analyzed by MSP can be used for the prediction of peritoneal micrometastases in patients with gastric cancer and assessment of the disease progress.

关 键 词：BNIP3 甲基化 胃癌 微转移 腹腔冲洗液 

分 类 号：R735.2[医药卫生—肿瘤]