通里攻下法防治豚鼠结石性胆囊炎动力学机制研究  

Kinetics Mechanism Study of Tongli Gongxia Formula Treating Guinea Pigs with Calculous Cholecystitis

作　　者：张栋梁 齐清会[2] 李毅[3] 周丽[4] 许士勇 范修峰 刘丹光 

机构地区：[1]镇江市中医院普通外科,江苏镇江212000 [2]大连医科大学第一附属医院普通外科,辽宁大连116011 [3]南京军区南京总医院解放军普通外科研究所,江苏南京210002 [4]大连大学附属新华医院肛肠科,辽宁大连116011

出　　处：《中华中医药学刊》2012年第10期2210-2212,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：江苏省中医药局医学科研指导项目(LB09128)

摘　　要：目的:探讨通里攻下方剂防治豚鼠结石性胆囊炎的动力学机制。方法:建立豚鼠结石性胆囊炎动物模型,制备消炎利胆汤含药血清,测定正常、模型、药物干预及Cajal间质细胞(Interstitial cells of cajal,ICC)损伤等各组胆囊肌条收缩活性。结果:模型组较正常组胆囊肌条收缩减弱、胆汁淤积,预防组较模型组、治疗组较对照组胆囊肌条收缩增强、胆汁淤积改善(P<0.05);含药血清可增强胆囊肌条收缩活性,呈浓度依赖性,ICC损伤胆囊肌条收缩活性减弱(P<0.05)。结论:胆囊收缩排空障碍、胆汁淤积为成石主要原因,通里攻下方剂可通过促进胆道动力防治胆石症,ICC在胆道动力中发挥作用。Objective ：To explore the kinetics mechanism of preventive and therapeutic effect of Tongli Gongxia formula on Calculous cholecystitis in guinea pigs. Method： The animal model of calculous cholecystitis was established, medicated serum of Xiaoyan Lidan Decoction was prepared, gallbladder smooth muscle~ activity of each group and the effect were ob- served. Result：There were significant differences between each control groups in gallstone formation rate and gallbladder smooth muscle activity （P 〈 0.05 ） ;the amplitude of contraction showed a dose -independent relationship with medicated serum in groups,the contraction of smooth muscle was nearly abolished when ICC was destroyed. Conclusion：Impaired bil- iary dynamics mechanism contributes to gallstone formation. Tongli Gongxia formula can treat cholelithiasis through the promotion of biliary dynamics. The damage of ICC may be an imoortant factor related to cholelithiasis.

关 键 词：通里攻下方剂 结石性胆囊炎 胆囊动力学 C ajal间质细胞 

分 类 号：R575.61[医药卫生—消化系统]