厚朴酚键合相HPLC测定纳尔平中的愈创木酚磺酸钾、对乙酰氨基苯酚及咖啡因  

Simultaneous determination of potassium guaiacolsulfonate,4-Acetamidophenol and caffeine on a magnolol-bonded silica gel stationary phase by HPLC

作　　者：陈红[1] 李来生[1] 张杨[1] 聂桂珍[1] 

机构地区：[1]南昌大学分析测试中心,南昌330047

出　　处：《分析试验室》2012年第11期67-71,共5页Chinese Journal of Analysis Laboratory

基　　金：江西省教育厅科技项目(GJJ11274)资助

摘　　要：采用厚朴酚键合硅胶固定相(MSP),建立了一种同时测定复方氨酚甲麻口服液中愈创木酚磺酸钾、对乙酰氨基苯酚和咖啡因的反相高效液相色谱法。流动相为甲醇-水(40:60,V/V),流速为0.8 mL/min,检测波长为270 nm。研究结果表明,在该色谱条件下,3种化合物能够实现基线分离,其峰面积与浓度呈良好线性关系,愈创木酚磺酸钾、对乙酰氨基苯酚和咖啡因的线性范围分别为5.50～27.50μg/mL、10.40～52.00μg/mL和1.14～22.80μg/mL,相关系数分别为0.9996、0.9999和0.9999。愈创木酚磺酸钾的平均回收率为92.4%～112.9%(RSD<4.6%),对乙酰氨基苯酚的平均回收率为101.5%～104.9%(RSD<0.90%),咖啡因的平均回收率为73.3%～87.8%(RSD<0.72%)。本方法能够满足实际样品的快速常规分析要求。A rapid HPLC method for simultaneous determination of potassium guaiacolsulfonate （PG）, 4-acetamidophenol and caffeine in the compound paracetamol and methylephedrine oral solution was developed with a magnolol-bonded silica gel stationary phase column （MPS）. The chromatographic analysis was carried out by using methanol-water （ 40 ： 60, V/V） as mobile phase at a flow rate of 0. 8 mL/min. The wavelength of quantitative analysis was set at 270 nm. These compounds were completely separated. The good linearity between the area of chromatographic peak and the concentration of each compound was observed. The linear ranges and the correlation coefficients of these compounds were 5.50 - 27.50 μg/mL （ r = 0. 9996 ）, 10. 40 - 52. 00μg/mL （r = 0. 9999 ） and 1.14 -22. 80μg/mL （r = 0. 9999 ）. The average recoveries of the spiked samples were 92.4% -112.9% （RSD 〈4.6%）, 101.5% -104.9% （RSD 〈0.90%） and 73.3% -87.8% （RSD 〈 0. 72% ）, respectively. The established method was convenient, fast and accurate.

关 键 词：反相高效液相色谱 厚朴酚键合硅胶固定相 复方氨酚甲麻口服液 愈创木酚磺酸钾 对乙酰氨基苯酚 咖啡因 

分 类 号：O657.7[理学—分析化学]