MRP2蛋白在食管鳞癌中的表达及其与化疗耐药的相关性  

The Expression of MRP2 Protein and its Correlation with the Chemoresistance in Esophageal Squamous Cell Carcinoma

作　　者：甘思远[1] 区增冠 孙艳芹[1] 陈娟[3] 陈莉熔 

机构地区：[1]广东医学院病理学教研室,广东湛江524023 [2]佛山市高明区中医院外科,广东佛山528511 [3]武警广东总队医院肿瘤科,广东广州510507

出　　处：《现代生物医学进展》2012年第25期4863-4865,共3页Progress in Modern Biomedicine

摘　　要：目的:探讨MRP2蛋白在食管鳞癌组织中的表达及其与食管癌化疗耐药的关系。方法:收集原发性食管鳞癌手术标本70例,采用免疫组织化学Envision法检测食管鳞癌组织及其癌旁组织中MRP2蛋白的表达情况,并采用MTT法检测食管鳞癌组织对临床常用化疗药物的敏感性,分析其表达与食管癌化疗耐药的关系。结果:70例食管鳞癌组织及其癌旁正常组织中的阳性表达率分别为58.6%及5.0%。MRP2蛋白在食管鳞癌组织中的阳性表达率明显高于癌旁正常食管组织(P<0.01)。食管鳞癌组织对环磷酰胺、5-氟尿嘧啶、吉西他滨、顺铂、卡铂、阿霉素、长春瑞滨、羟喜树碱等化疗药物的敏感性与其相应癌组织中MRP2表达明显相关(P<0.01)。结论:MRP2的表达与食管鳞癌对多种化疗药物耐药有较好的相关性,推测食管鳞癌组织中MRP2的高表达可能对化疗耐药性的发生发展具有促进作用。Objective： To investigate the expression of MRP2 protein and its correlation with the chemoresistance in esophageal squamous cell carcinoma.Methods： 70 cases with esophageal squamous cell carcinomas were chosen.The expression of MRP2 in esophageal squamous cell carcinomas and pericarcinoma tissues was detected with immunohistochemical Elivision TM plus non-hiotin technique.The chemoresistace was assayed by MTT method.Results： The positive expression rates of MRP2 protein in esophageal squa-mous cell carcinomas and pericarcinoma tissues were 58.6 % and 5.0 % respectively.The positive expression rates of MRP2 protein in esophageal squamous cell carcinomas were significantly higher than that in pericarcinoma tissues（P0.01）.The expression of MRP2 was negatively correlated to the chemoresistance,except mitomycin and paclitaxel.Conclusion： The MRP2 protein is overexpressed and the chemoresistance of the chemotherapeutics probably correlates with the MRP2 expression in esophageal squamous cell carcinomas.

关 键 词：食管鳞状细胞癌 免疫组织化学 MTT 多药耐药相关蛋白2 

分 类 号：R735.1[医药卫生—肿瘤]