外源性硫化氢对失血性休克复苏大鼠心肌细胞NF-κB活性的影响  

Effect of exogenous hydrogen sulfide on myocardial NF-kB signaling pathway in rats with hemorrhagic shock

作　　者：高操[1] 杨璐[2] 沈江[1] 洪涛[1] 张弦[3] 丁倩[2] 高昌俊[2] 柴伟[2] 

机构地区：[1]苏州大学附属第三医院麻醉科,江苏省常州市213000 [2]第四军医大学唐都医院麻醉科 [3]解放军第305医院药局

出　　处：《中华麻醉学杂志》2012年第8期985-987,共3页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金项目（面上项目）（30872444）

摘　　要：目的评价外源性硫化氢对失血性休克复苏大鼠心肌细胞NF-kB活性的影响。方法清洁级成年雄性SD大鼠40只，体重250—300g，采用随机数字表法，将其随机分为4组（n=10）；假手术组（Sham组）、假手术＋硫氢化钠组（Sham＋NariS组）、失血性休克组（HS组）和失血休克＋硫氢化钠组（us＋NaHS组）。HS组采用股动脉放血的方法制备大鼠失血性休克模型；HS＋Nails组失血性休克后复苏前10min腹腔注射NaHS28ttmol／kg，Sham＋NaHS组同时点腹腔注射28μmol／kgNaHS。于放血即刻、放血后1．5、2、3、4和6h时记录大鼠MAP，随后处死大鼠取心脏，采用Westemblot法测定心肌细胞plKKβ、pIkBa、pNF-kBp65和HMGBl表达，光镜和电镜下观察心肌组织超微结构。结果与Sham组和Sham＋NaHS组相比，HS组和HS＋NaHS组MAP降低，心肌细胞plKKβ、pIkBa、pNF-kBp65和HMGBl表达上调（P〈0．05）；与HS组相比，HS＋NaHS组MAP升高，心肌细胞plKKβ、pIkBa、pNF-kBp65和HMGBl表达下调（P〈0．05）。HS＋NaHS组心肌病理学损伤较HS组减轻。结论外源性硫化氢可抑制NF-kB活性，减轻炎性反应，从而减轻失血性休克复苏大鼠的心肌损伤。Objective To investigate the effect of exogenous hydrogen sulfide on myocardial NF-kB sig- naling pathway in rats with hemorrhagic shock （HS） .Methods Forty adult male rats, weighing 250-300 g, were randomly divided into 4 groups （ n = 10 each） ： sham operation group （Sham group）, sham operation ＋ NariS group （Sham ＋ NariS group）, HS group and HS ＋ NariS group. HS was induced by withdrawing blood from the femoral artery. After HS, NariS 28 μmol/kg was injected intraperitoneally at 10 min before resuscitation in groups HS ＋ NariS and Sham ＋ NariS. MAP was monitored and recorded at 0, 1.5, 2, 3, 4 and 6 h after blood-letting. The rats were then sacrificed and hearts were removed for determination of phosphorylated IKKβ （pIKKβ）, IkBa （pIkBa）, NF-kB p65 （pNF-kB p65） and high-mobility group box 1 （HMGB1） and for examination of myocardial ultrastructure with light arid electron microscope. Results Compared with Sham and Sham ＋ NaSH groups, MAP was significantly decreased and the expression of pIKKβ, pIkBa, pNF-kB p65 and HMGB1 was up-regulated in HS and HS ＋ Naris groups （ P 〈 0.05）. Compared with HS group, MAP was significantly increased and the expres- sion of pIKKβ, pIkBa, pNF-kB p65 and HMGB1 was down-regulated in HS ＋ NariS group （P 〈 0.05）. The pathologic changes were attenuated in HS ＋ NariS group compared with group HS. Conclusion Exogenous hydro- gen sulfide can attenuate myocardial injury induced by HS through inhibition of NF-kB signaling pathway and re- duction of inflammatory response.

关 键 词：硫化氢 休克 出血性 心肌再灌注损伤 NF-KB 

分 类 号：R614[医药卫生—麻醉学]