P-gp与GST-π在鼻咽癌多药耐药细胞中的表达及其意义  

Expression of P-gp and GST-π in multi-drug resistance cells in nasopharyngeal cancer and their clinical significances

作　　者：刘津[1] 覃继新[1] 冯云[2] 

机构地区：[1]右江民族医学院附属医院耳鼻喉科,广西百色533000 [2]右江民族医学院附属医院神经内科,广西百色533000

出　　处：《右江民族医学院学报》2012年第5期601-603,共3页Journal of Youjiang Medical University for Nationalities

基　　金：广西壮族自治区卫生厅科研课题(合同号:Z2011141);广西医学科学实验中心开放基金专项项目(合同编号:KFJJ2010-62);百色市科学研究与技术开发计划课题(合同编号:百科计1001003)

摘　　要：目的比较P-糖蛋白(P-gp)与谷胱甘肽S转移酶π(GST-π)在鼻咽癌细胞TW03及其多药耐药细胞TW03/DDP中的表达差异,进一步探讨鼻咽癌多药耐药的机制。方法制备鼻咽癌细胞TW03及其多药耐药细胞TW03/DDP的细胞爬片,利用免疫细胞化学技术,检测P-gp与GST-π在两种细胞中的表达情况。结果 GST-π在TW03/DDP中表达明显高于TW03(P<0.01),而P-gp在TW03/DDP中表达明显低于TW03(P<0.01)。结论 GST-π可能是TW03/DDP导致多药耐药中的重要原因之一,P-gp降低的原因需要进一步研究。Objective To compare the expression differences of P- glucoprotein （P- gp） and glutathione S-transferase 7r （GST- π） between nasopharyngeal cancer cells TW03 and its multi- drug resistance ceils TW03/DDP, zncl to further studv the multi - dru~ resistance mechanism of nasot3harvnzeal cancer. Methods The cell slide preparation for nasopharyngeal cancer cells TW03 and its multi- drug resistance cells TW03/DDP was performed. An immunocytochemistry technique was used to detect the expression of P - gp and GST- rt in the two mentionedabove cells. Results The expression of GST - πin TW03/DDP was statistically higher than that in TW03 （ P 〈0.01）, while the expression of P - gp in TW03/DDP was statistically lower than that in TW03 （ P 〈 0.01）. Con- clusion GST - zt may play an important role in TW03/DDP - induced multi - drug resistance. The mechanism ofdecreased P - gp needs further study.

关 键 词：多药耐药相关蛋白类 谷胱甘肽转移酶 P糖蛋白 鼻咽肿瘤 

分 类 号：R739.63[医药卫生—肿瘤]