狂犬病病毒SYBR-GreenⅠ实时荧光定量PCR检测方法的建立  

Establish of SYBR-GreenⅠFluorescent Quantitative PCR Method for Detecting Rabies Virus

作　　者：陆专灵[1,2] 何晓霞[1,2] 谢琳娟[1,2] 梁湘[1,2] 唐海波[1,2] 罗扬[1,2] 罗廷荣[1,2] 

机构地区：[1]广西大学亚热带农业生物资源保护与利用国家重点实验室,南宁530005 [2]广西大学动物科学技术学院,南宁530005

出　　处：《基因组学与应用生物学》2012年第5期441-446,共6页Genomics and Applied Biology

基　　金：国家重点基础研究发展计划(973计划);狂犬病毒分子流行病学及种间进化特点课题(2005CB523000)资助

摘　　要：狂犬病病毒为不分节段单链负股的RNA病毒,属弹状病毒科狂犬病病毒属。世界上几乎所有国家都有狂犬病发生,狂犬病病毒能够使所有温血动物发病致死,死亡率高达100%。本研究根据GenBank中的狂犬病病毒M基因序列,选择保守区域设计引物,通过对SYBR-GreenⅠ实时荧光PCR反应条件进行优化,建立了用于检测狂犬病病毒的SYBR-GreenⅠ实时荧光PCR方法。结果显示,建立的狂犬病病毒实时荧光定量PCR方法,具有特异性强、灵敏度高、重复性好的优点,是开展狂犬病的临床检测的有力工具。Rabies virus is a non-segmented, negative-strand RNA virus, belonging to the genus of lyssavirus of the rhabdoviridae family. Rabies distributes all over the world, all warm-blood animal are susceptible to rabies virus with 100% mortality rate. In this study, the conserved regions of rabies virus M genes chosen from GenBank were used to design primers for fluorescent quantitative PCR, after optimized the reaction condition, the SYBR-GreenⅠfluorescent quantitative PCR method for detecting rabies virus was established and used to detect rabies virus. The results showed SYBR-GreenⅠfluorescent quantitative PCR was high specific, high sensitivity, good reproducible, which might be a useful tool for detecting rabies virus.

关 键 词：狂犬病病毒 SYBR-GreenⅠ实时荧光定量PCR 

分 类 号：S855.3[农业科学—临床兽医学]