基于线粒体COⅠ及Cyt b基因的7种龙虾类分子系统关系  

Phylogenetic Relationship of Seven Lobster Species Based on Mitochondrial COⅠ and Cyt b Genes Sequences

作　　者：姚维[1] 刘楚吾[1] 刘丽[1] 

机构地区：[1]广东海洋大学水产学院,南海水产经济动物增养殖广东普通高校重点实验室,湛江524025

出　　处：《基因组学与应用生物学》2012年第5期473-480,共8页Genomics and Applied Biology

基　　金：广东省科技计划项目(No.2010B020308004);国家科技支撑计划项目(No.2007BAD29B03);湛江市科技攻关项目(No.2007C09006)共同资助

摘　　要：运用PCR法扩增湛江沿海海域7种龙虾的线粒体COⅠ和Cytb基因并对其序列进行分析,以分析7种龙虾的分子系统关系。从7种龙虾中扩增到的COⅠ基因片段长度均为650bp,共存在224个核苷酸位点变异,变异率为35.22%,简约信息位点161个;扩增到的Cytb基因片段长度均为536bp,共存在148个核苷酸位点变异,变异率为29.48%,简约信息位点66个。所有的扩增序列中,没有发现碱基的缺失以及插入,序列中的转换大于颠换,且碱基替换多发生于密码子的第3位。以帝加洛真龙虾(Palinurus delagoae)、普通真龙虾(Palinurus elephas)、吉氏真龙虾(Palinurus gilchristi)为外群,对COⅠ和Cytb两个基因序列采用最大简约法(maximum parsimony,MP)和贝叶斯推论法(bayesian inference,BI)构建龙虾类分子系统树。结果显示:日本龙虾和密毛龙虾亲缘关系比较近,而其它5种龙虾与真龙虾属的3种关系较近,与传统的分类存在一定分歧。Mitochondrial COⅠ and Cyt b genes of seven lobster species in Zhanjiang coastal water were amplified by PCR sequencing and their sequences were analyzed. The results showed that the COⅠgene segments length of seven lobster species were 650 bp. There are 161 parsimony-informative sites and 224 nucleotide variation sites with the variation rate is 35.22%. The length of Cyt b gene segments are 536 bp, containing 148 nucleotide variatio sites with a variation rate of 29.48% and 66 parsimony-informative sites. In all sequences, there is no indel, and conversion is greater than transversion. The substitution more often occurs in the third codon. We used Maximum Parsimony （MP） and Bayesian Inference （BI） to study the union sequences of COⅠ and Cyt b and construct lobster＇s molecular phylogenetic tree. The results showed that Palinurus delagoae, Palinurus elephas and Palinurus gilchristi are outgroup. The genetic relationship of P. Japonicus and P. Penicillatu is near, same as the 3 Palinurusand species and the 5 Panulirus species, which is different with traditional classification system.

关 键 词：龙虾 线粒体 COⅠ CYT b 分子系统树 

分 类 号：S917.4[农业科学—水产科学]