岷山红三叶草异黄酮合酶基因的克隆及序列分析  

Cloning and sequence analysis of isoflavones synthase gene from Trifolium Pratense Minshan

作　　者：胡焕焕[1] 刘瑞[2] 姬国杰[1] 李卫东[1] 丰慧根[1] 

机构地区：[1]新乡医学院生命科学技术系,河南新乡453000 [2]新乡医学院三全学院,河南新乡453000

出　　处：《食品工业科技》2012年第21期178-180,共3页Science and Technology of Food Industry

基　　金：河南省科技厅重点支持项目(102102310189);河南省教育厅立项项目(2009A180014);新乡医学院研究生科研创新支持项目(YJSCX201116Y)

摘　　要：从岷山红三叶克隆异黄酮合酶基因,并对序列进行比对和进化分析。建立岷山红三叶愈伤组织培养体系,采用RT-PCR扩增从愈伤组织总RNA中分离了异黄酮合酶基因,并将其克隆到pGEM-T Easy载体,测序,得到全长1575bp的片段。序列分析表明,异黄酮合酶基因含1575个核苷酸,和大豆等其他植物中异黄酮合酶具有很高的同源性。For making good use of the specific biological function of isoflavone.A cDNA fragment from full length IFS gene was amplified by polymerase chain reaction from total RNA of callus Trifolium Pratense Minshan, and it was sequenced after cloned into pGEM-T Easy vectors.A cDNA fragment of 1575bp from full length IFS gene was got. The sequencing results indicated that the cloned fragment of IFS contained 1575 nucleotides, and shared a sequence homology of 99% with that from Genbank accession.

关 键 词：岷山红三叶 异黄酮合酶 基因克隆 序列分析 

分 类 号：Q943.2[生物学—植物学]