生长因子对螺旋藻混合营养生长及藻胆蛋白含量影响的研究  

The influence of positive growth factor on mixotrophic growth of S. platensis and the content of phycobiliproteins

作　　者：谯顺彬[1] 董汝晶[1] 田辉 张义明[1,3] 罗芳[1] 陶希芹 

机构地区：[1]贵州工业职业技术学院,贵州贵阳550008 [2]贵州茅台酒厂(集团)习酒有限责任公司,贵州习水564622 [3]贵州省发酵工程与生物制药重点实验室,贵州贵阳550003

出　　处：《食品工业科技》2012年第21期254-257,407,共5页Science and Technology of Food Industry

基　　金：国家自然科学基金项目(39860004);贵州省科学技术基金项目(黔科合J字[2010]2068号)

摘　　要：本文考察了维生素B12(VB12)、精氨酸(Arg)和萘乙酸(NAA)三个生长因子对螺旋藻混合营养培养的影响,并对藻体干重(DW)及藻胆蛋白(PCP)的含量进行检测。实验结果表明:VB12对螺旋藻生长以及藻胆蛋白的积累均有较大影响,NAA对螺旋藻生长影响较大,而Arg对螺旋藻中藻胆蛋白含量影响较大;在单因素实验的基础上,利用响应面法(RSM)确定三个生长因子的最佳水平:VB12、Arg和NAA水平配比为0.08、45、0.3mg/L,此时藻体干重与藻胆蛋白含量分别增加了20.2%和31.9%;以VB12、Arg和NAA为自变量,建立了藻体干重和藻胆蛋白为响应值的二次多项式数学模型。In this paper, three critical growth factors were selected to research the influence for the growth of Spirulina platensis, employing the response surface methodology to optimize the culture conditions of biomass concentration and phycobiliprotein accumulation.Three critical factors selected for investigation were VB12 ,Arg and NAA.Firstly, single-factor experiment was used to determine the range which the biomass concentration of Spirulina platensis had the obvious improvement. Secondly, the response surface methodology was used to optimize the three factors, and to build up a quadratic regression equation with dry weight and PCP as the response,VB12 ,Arg and NAA as independent, respectively.Finally, after optimization of the experiment showed that, the best level was VB12, Arg and NAA 0.08,45,0.3mg/L, dry weight and phycobiliprotein content, an increase of 20.2% and 31.9%, respectively.

关 键 词：螺旋藻 混合营养培养 生长因子 藻胆蛋白 

分 类 号：Q939.97[生物学—微生物学]