HPLC法同时测定不同品种溪黄草中3种三萜类成分的含量  

HPLC simultaneous determination of three triperpenoids components in Linearstripe Rabdosia Herb from different species

作　　者：吴泽青[1] 帅欧[1] 林励[1] 邱戊盛 欧先涛 吴攀创 

机构地区：[1]广州中医药大学中药学院,广东广州510006 [2]广东万年青制药有限公司,广东汕头515064

出　　处：《食品工业科技》2012年第21期296-299,共4页Science and Technology of Food Industry

摘　　要：目的:建立同时测定溪黄草中科罗索酸、齐墩果酸及熊果酸含量的高效液相色谱法。方法:采用Synergi 4u Fusion RP 80A C18(250mm×4.6mm,5μm,Phenomenex company)色谱柱;流动相:甲醇(A)-0.2%磷酸水(B)(V∶V=9∶1);等度洗脱;流速:0.6mL·min-1;检测波长:210nm;柱温30℃。结果:科罗索酸、齐墩果酸及熊果酸分别在0.111～1.111mg/mL(r=0.9999)、0.026～0.256mg/mL(r=0.9999)、0.307～3.067mg/mL(r=0.9999)范围内线性关系良好,平均回收率分别为97.72%、97.08%和97.46%(n=6),RSD分别为1.24%、1.62%和1.25%。结论:本法快速、简便、准确,重复性好,可用于溪黄草3种主要成分定量分析和质量控制。Objective：To establish an HPLC method for simultaneous determination of 2α-hydroxy-ursolic acid, oleanolic acid and ursolic acid in Linearstripe Rabdosia Herb from different species.Methods：The HPLC system consisting of Synergi 4u Fusion RP 80A C18 column （250mm ×4.6mm, 5μm, Phenomenex company） and a solution system of methanol-phosphoric acid（V：V = 9：1 ）as the mobile phase with isocratic elution was adopted.The flow rate was set at 0.6mL·min-1 ,the detection wavelength was 210nm and the column temperature was 30℃.Results： The linear ranges of 2cc-hydroxy-ursolic acid ,oleanolic acid and ursolic acid were 0.111-1.111mg/mL（ r =0.9999）, 0.026- 0.256mg/mL（ r = 0.9999） ,0.307-3.067mg/mL（ r = 0.9999 ） respectively.Conclusion ： The average recoveries （ n =6）were 97.72% （RSD = 1.24% ）,97.08% （RSD = 1.62% ）,97.46% （RSD = 1.25% ） respectively.The method was quick ,simple and accurate with good reproducibility and could be used for determination and quality control of the three main components in Linearstripe Rabdosia Herb.

关 键 词：溪黄草 三萜 高效液相色谱法 测定 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]