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作 者:马燕[1] 蔺艳[1] 孙丽萍[1] 梁芳[1] 董爱香[1]
出 处:《草业科学》2012年第10期1569-1573,共5页Pratacultural Science
摘 要:试验利用多次混合选择、常规杂交育种及辐射育种和离子注入的方法在北京地区进行美丽月见草(Oeno-thera speciosa)抗寒性育种。结果表明,多次混合选择育种可以提高美丽月见草的抗寒性,经4年选择使越冬率从4.7%提高到6.5%;美丽月见草与两种抗寒月见草品种[金日光月见草(O.tetragona)和大花月见草(O.erythrosepala)]进行有性杂交均无结实,经多年杂交试验未获得有效种子;60 Co-γ辐射大粒种子,中辐射剂量200Gy,成活率44.4%的美丽月见草越冬率为37.5%,Si离子注入小粒种子,中能量35 MeV、注量5×1010P.cm-2,成活率73.1%的美丽月见草越冬率为55.3%,处理之前的越冬率仅为6.5%。试验表明,Si离子注入可明显提高美丽月见草的抗寒性。Cold-resistance breeding for Oenothera speciosa in Beijing was carried out.Many times of bulk selection breeding,conventional cross breeding,radioactive breeding and ion implantation breeding were used in this study.Results showed that many times of bulk selection breeding could enhance cold resistance of O.speciosa which made the surviving rate of overwintering raised from 4.7% to 6.5% in four years.Cross breeding was performed with O.speciosa and another two cold-resistance species,but there were no hybrid seeds during many years' study.With the treatment of radiation dosage 200 Gy and 60Co-γ-radioactive to big seeds,the surviving rate of the seeds was 44.4%,while the surviving rate of overwintering could achieve 37.5%.The surviving rate of O.speciosa was 73.1% with the treatment of energy 35 MeV,injection dosage of 5×1010 P·cm-2,and Si ion implantation to small seeds,and the surviving rate of overwintering could achieve 55.3%.Notably,Surviving rate of overwintering was 6.5% before treatment.The best method to enhance cold resistance of O.speciosa is Si ion implantation,which made the highest surviving rate of overwintering in this study.
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