血管内皮生长因子936^＊T／C基因多态性与胃癌的关系  

Relationship between VEGF Gene 936^＊T/C Polymorphism and Gastric Cancer

作　　者：林伟[1] 陈金坤[1] 邱仙土[1] 陈凯[1] 

机构地区：[1]莆田学院附属医院普通外科,福建莆田351100

出　　处：《莆田学院学报》2012年第5期33-35,74,共4页Journal of putian University

基　　金：福建省自然科学基金资助项目(2012J01430);莆田市科技局基金资助项目(2010S10-9)

摘　　要：研究血管内皮生长因子(VEGF)936*T/C基因多态性与胃癌之间的关系,了解该基因多态性对胃癌生成及发展的影响。采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测胃癌患者和健康者外周血的VEGF936*T/C基因型。结果,胃癌患者外周血中VEGF936*T/C基因型或等位基因与健康者相比无差异(精确概率法计算基因型P=0.226;卡方检验等位基因x2=2.934,P=0.087)。Ⅲ、Ⅳ期病理分期患者C/C基因型和C等位基因比例(66.7%和82.0%)明显大于Ⅰ、Ⅱ期(12.9%和1.2%),两者差异有统计学意义(基因型:x2=14.215,P=0.000;等位基因:x2=28.430,P=0.000)。结果表明,VEGF936*C/C基因多态性与胃癌的生成无关,而与胃癌的进展相关。Objective The study was to investigate the relationship between VEGF gene 936^＊T/C polymorphism and gastric cancer. Methods VEGF936 ^＊T/C genotypes was detected by PCR-RFLP in patients with gastric carcinoma and healthy controls. Results There was no significant difference in the gentype of VEGF936^＊T/C or C allele between the patients and the control group （ genotype P=0.226; C allele x^2=2.934, P=0.087 ）. The C/C genotype and C allele in patients at Ⅲ, Ⅳ staged （ 66.7% and 82.0% ） were more frequently found than in those with Ⅰ, Ⅱ staged （12.9% and 1.2%）, so there was significant difference （C/C genotype; （x^2=14.215, P=0.000. C allele x^2-28.430, P=0.000 ）. Conclusions VEGF936＊C/C genotype is not related to the development of gastric carcinoma, but they both are related to the growth of the carcinoma.

关 键 词：VEGF 胃癌 基因多态性 

分 类 号：R735.2[医药卫生—肿瘤]