三峡兰陵溪杉木马尾松混交林土壤DNA空间异质性分析  

Spatial Variability of Soil DNA in China Fir and Masson Pine Mixed Forest in Three Gorger Reservoir Areas

作　　者：林英华[1,2] 汪来发[2] 卢萍[1,2] 田晓堃[2,3,4] 肖文发[2] 

机构地区：[1]中国林业科学研究院湿地研究所,北京100091 [2]中国林业科学研究院森林生态环境与保护研究所,国家林业局森林保护学重点实验室,北京100091 [3]中国林业科学研究院亚热带林业研究所,浙江富阳311400 [4]华中农业大学园艺林学学院,湖北武汉430070

出　　处：《林业科学研究》2012年第5期664-669,共6页Forest Research

基　　金：中央级公益性科研院所基本科研业务费专项资金(CAFRIF2007009;CAFINT2010K08)

摘　　要：Soil samples were collected to a depth of 20 cm using a 3.0 m × 3.0 m grid in 900 m 2(30.0 m ×30.0 m) plots for analysis of spatial patterns of soil in undisturbed area of China fir(Cunninghamia lanceolata) and Masson pine(Pinus massoniana) mixed forest in the Three Gorger Reservoir Areas.The investigation data showed that the average of soil DNA content was 922.71 μg·g-1 water-free soil,the maximal value was 6 761.30 μg·g-1 water-free soil,and the less value 58.52 μg·g-1 water-free soil.The geostatistical analysis indicated that the spatial distribution characteristic was not obviously reflected by the liner graphs while samples were randomly distribution and independent each other.The separation distance among samples was related to precision of soil DNA content,the effects of non-structure factors(environment factors,disturbed factors) on spatial correlation structure would disappeared due to the separation distance in excess of 18.84 m,and the spatial correlation of soil DNA faded down in plots.Spatial variability in soil DNA in the region was mostly contributed by the influencing factors including soil nitrogen,soil organic matter and content of soil water.Soil samples were collected to a depth of 20 cm using a 3.0 m × 3.0 m grid in 900 m 2（30.0 m ×30.0 m） plots for analysis of spatial patterns of soil in undisturbed area of China fir（Cunninghamia lanceolata） and Masson pine（Pinus massoniana） mixed forest in the Three Gorger Reservoir Areas.The investigation data showed that the average of soil DNA content was 922.71 μg·g-1 water-free soil,the maximal value was 6 761.30 μg·g-1 water-free soil,and the less value 58.52 μg·g-1 water-free soil.The geostatistical analysis indicated that the spatial distribution characteristic was not obviously reflected by the liner graphs while samples were randomly distribution and independent each other.The separation distance among samples was related to precision of soil DNA content,the effects of non-structure factors（environment factors,disturbed factors） on spatial correlation structure would disappeared due to the separation distance in excess of 18.84 m,and the spatial correlation of soil DNA faded down in plots.Spatial variability in soil DNA in the region was mostly contributed by the influencing factors including soil nitrogen,soil organic matter and content of soil water.

关 键 词：土壤DNA 空间分布 插值法 多元回归 湖北秭归 

分 类 号：S714.3[农业科学—林学] S791.246