5-氮杂-2’-脱氧胞苷对伯基特淋巴瘤NAMALWA细胞株增殖及阳性调控区锌指蛋白1α基因表达的影响  

Effect of 5-Aza-CdR on proliferation and PRDM1α gene expression in Burkitt＂ s lymphoma cell line

作　　者：刘林[1] 张凤[1] 

机构地区：[1]安徽省蚌埠医学院第一附属医院血液科,233000

出　　处：《白血病．淋巴瘤》2012年第10期607-610,共4页Journal of Leukemia & Lymphoma

基　　金：安徽省卫生厅基金（20088002）

摘　　要：目的探讨DNA甲基化抑制剂5．氮杂-2’-脱氧胞苷（5-Aza-CdR）对伯基特淋巴瘤NAMALWA细胞株增殖及抑癌基因阳性调控区锌指蛋白10[（PRDM101）表达的影响。方法四甲基偶氮唑蓝（MTr）法检测5-Aza．CdR对NAMALWA细胞株增殖的影响，SYBRGreen相对定量反转录聚合酶链反应方法检测PRDM10α基因表达水平。结果5-Aza．CdR可抑制NAMALWA细胞的增殖，且表现为浓度依赖性，在终浓度为0．01、0．0625、0．1、0．125、0．25、0．5、1、2和4μmol／L时对NAMALWA细胞株的抑制率分别为21．93％、39．23％、47．69％、50．37％、53．54％、57．72％、62_31％、65．68％和67．87％，且不同药物浓度间吸光度值比较，差异有统计学意义（均P〈0．01）。同时，5-Aza—CdR能使NAMALWA细胞株中PRDM1α重新表达，0．5、1、2μmol／L加药组与对照组比较，△Cf之间差异有统计学意义（P〈0．05）。结论DNA甲基化抑制剂5-Aza—CdR能显著抑制伯基特淋巴瘤NAMALWA细胞株增殖，可能与其诱导的PRDM1α基因的去甲基化和PRDM1α的再表达有关。Objective To explore the effect of DNA methylation inhibitor 5-aza-2＇-deoxycytidine （5-Aza-CdR） on proliferation and tumor suppressor gene PRDMlet expression in Burkitt＇ s lymphoma cell line NAMALWA. Methods MTF was used to study the impact of DNA methylation inhibitor on proliferation in NAMALWA cells, and comparative real-time reverse transcription-polymerase chain reaction （RQ-PCR） with SYBR Green assay was used to detect PRDM1α gene expression. Results MTT results showed that 5-Aza- CdR inhibited proliferation of NAMALWA cells in a dose-dependent manner. After treated with 5-Aza-CdR for 72 h at 0.01, 0.0625, 0.1, 0.125, 0.25, 0.5, 1, 2 and 4 μmol/L, the inhibition rates were 21.93 %, 39.23 %, 47.69 %, 50.37 %, 53.54 %, 57.72 %, 62.31%, 65.68 %, 67.87 %, respectively, and the differences of absorbance among the groups were also statistically significant （all P 〈 0.01）. Meanwhile, 5-Aza-CdR could induce the re-expressi0n of PRDM1α, and ACt of 0.5, 1, 2 μmol/L groups showed significant differences compared with control group （all P 〈 0.05）. Conclusion DNA methylation inhibitor 5-Aza-CdR can significantly inhibit the proliferation of Burkitt＇ s lymphoma cell line NAMALWA, and the possible mechanism of this inhibition may be induction of demethylation and re-expression of PRDMlet.

关 键 词：DNA甲基化抑制剂 伯基特淋巴瘤 NAMALWA细胞株 阳性调控区锌指蛋白 1α基因 细胞增殖 

分 类 号：R733.1[医药卫生—肿瘤]