EGFRvⅢ的表达对吉非替尼作用卵巢癌SKOV3细胞敏感性的影响  

Effect of EGFRvⅢ expression on sensitivity of ovarian cancer SKOV3 cell after Gefitinib treatment

作　　者：张莉[1] 包乐纹[2] 

机构地区：[1]天津医科大学附属肿瘤医院妇瘤科,天津300060 [2]天津市肿瘤防治重点实验室,天津300060

出　　处：《重庆医科大学学报》2012年第11期940-943,共4页Journal of Chongqing Medical University

摘　　要：目的:探讨EGFRvⅢ的表达与卵巢癌SKOV3细胞对化疗药吉非替尼敏感性之间的关系,为临床治疗并合理选用抗肿瘤药物提供参考。方法:将质粒pcDNA4/TO-EGFRvⅢ稳定转染入SKOV3细胞,并用zeocin抗生素筛选出稳定表达EGFRvⅢ蛋白的细胞株(命名为SKOV3-EGFRvⅢ),用MTT法检测转染稳定株和原始株在吉非替尼作用下细胞的存活能力。然后,以吉非替尼分别对2株细胞建立的裸鼠异体肿瘤模型进行治疗,通过测量肿瘤体积大小评价肿瘤模型对吉非替尼的敏感性。结果:通过Western blot鉴定,SKOV3-EGFRvⅢ稳定株建立成功;MMT法结果表明SKOV3-EGFRvⅢ细胞在吉非替尼的作用下,其死亡率低于原始细胞株,且吉非替尼对SKOV3-EGFRvⅢ细胞建立的裸鼠肿瘤模型的抑制效果明显低于原始细胞株。结论:EGFRvⅢ的过表达导致卵巢癌SKOV3细胞及其体外移植肿瘤模型对化疗药吉非替尼的敏感性减弱。Objective：To determine the relationship between EGFRvⅢ expression and sensitivity of SKOV3 cell to Gefitinib and to give rational guidance to clinical medication.Methods：Plasmid pcDNA4/TO-EGFRvⅢ was stably transfected into SKOV3 cell and the cell strains stably expressing EGFRvⅢ were screened out by zeocin antibiotics and named SKOV3-EGFRvⅢ.MTT was used to assess the viability of SKOV3-EGFRvⅢ cell and SKOV3 cell treated by Gefitinib.Nude mice allograft tumor models bearing SKOV3 and SKOV-EGFRvⅢ were treated by Gefitinib.The sensitivity of the tumor model to Gefitinib was assessed by measuring the tumor size.Results：Results of Western blot indicated that SKOV3-EGFRvⅢ cell strain was successfully established.According to the results of MMT,after being treated by Gefitinib,the mortality was lower in SKOV3-EGFRvⅢ cell than in SKOV3 cell and the inhibition effect of Gefitinib was less intense on tumor model carrying SKOV3-EGFRvⅢ cell than on that carrying SKOV3.Conclusion： Over expression of EGFRvⅢ in ovarian cancer SKOV3 cell results in its decreased sensitivity to Gefitinib.

关 键 词：EGFRvⅢ SKOV3细胞 敏感性 吉非替尼 

分 类 号：R737[医药卫生—肿瘤]